摘要
目的通过制备大鼠离体心脏灌注模型，探讨模型制备的方法及注意事项。方法健康成年大鼠20只，雌雄不限，取离体心脏，制备Langendorff模型及离体工作心脏灌注模型。结果实验中成功18例（成功率90％）。心脏均能持续稳定搏动，心脏收缩有力，左室内压为7．1～10．5kPa，处于生理范围（6．3～10．3kPa），心率250-300／min，心律齐，心脏可平稳跳动6h以上。结论Langendorff模型及离体工作心脏灌注模型操作方便、稳定，是比较符合生理特性的离体心脏灌注模型。
Objective To establish isolated rat heart model. Methods Twenty SD rats were divided into 5 groups randomly,isolated rat heart I.angendorff model and isolated working heart model were established. Results Eighteen models were established successfully （90%）. The pressure in left ventricle was 7.1-10.5 kPa,and the hearts could beat stably for more than 6 h. Conclusion In this study,we established isolated rat heart Langendorff model and isolated working heart model, which were very convenient and stable.
出处
《海军总医院学报》
2006年第3期133-135,共3页
Journal of Naval General Hospital of PLA
关键词
离体心脏
灌注模型制备
Isolated rat heart
Perfusion model preparation
