摘要
目的:体外试验研究三氧化二砷(As2O3)对兔晶体上皮细胞(RLECs)增殖的影响。方法:取第3代RLECs,分为正常组和加入不同浓度三氧化二砷的实验组,分别于作用24小时、72小时和5天后观察各组LECs的形态学改变,用MTT比色法测定As2O3对LECs的影响。结果:As2O3作用于LECs24小时的IC50为3.101μmol/L,72小时的IC50为1.743μmol/L,5天的IC50为1.094μmol/L。结论:As2O3为剂量依赖性和时间依赖性药物,对体外培养的LECs增殖有明显抑制作用。
Objective： To evaluate The effect of arsenic trioxide on the proliferation of rabbit lens epithelial cells in vitro. Methods： Divide 3-passage RLECs into joined control group and different concentrations of arsenic trioxide groups for 24 hours, 72 hours and 5 days, the effects of RLECs were measured with methyl thiazolyl tetrazolium（MTT） assay and the apoptosis of cells were observed. Results： The IC50 of arisenic trioxide exposed to RLECs for 24 hours was 3.101μmol/L, that of 72 hours was 1.743μmol/L and that of 5 days was 1.094μmol/L. Conclusion： Arsenic trioxide was dose-dependent and time-dependent inhibitor, and inhibited the proliferation of RLECs in vitro effectively.
出处
《中国伤残医学》
2006年第3期19-21,共3页
Chinese Journal of Trauma and Disability Medicine
基金
黑龙江省卫生厅基金项目(编号2005-141)
关键词
三氧化二砷
晶体上皮细胞
Arsenic trioxide
Lens epithelial cells
