摘要
以提莫菲维小麦基因组DNA为模板,采用设计的小麦种子醇溶蛋白的保守引物进行PCR扩增,扩增产物插入pMD-18T载体,并转化到大肠杆菌DH5α中,对阳性克隆进行测序。结果表明,扩增产物长度为1 002 bp,包含一个完整的284个氨基酸的编码区,基因库登录号为DQ861428。序列比对表明,该序列为-αgliadin基因家系成员。利用-αgliadin基因的编码氨基酸序列建立系统树,分析表明该序列与栽培小麦供体物种一粒小麦的-αgliadin基因聚在一大类中,因而被定位在提莫菲维小麦的A染色体组上。
The α-gliadin gene sequences from the genomic DNA from Triticum tirnopheevii was done with the PCR based on the conserved primers published. The PCR product was inserted into pMD-18T vector and transferred into E. coli DHSa. The positive clones with target insertion of PCR product were sequenced, The results showed that the PCR product contained a length of 1 002 bp including a coding region of 284 amino acids, with gene-bank accession number DQ861428. BLAST search showed that it was a member of α-gliadin super-family. The phylogenetic trees based on the amino acid sequence ofα-gliadin gene indicated that the present DQ861428 was clustered in the clade of T. monococcum,and the fact indicated that it was located on the A genome of T. timopheevii. The utilization of the T. timopheevii derived seeds storage protein genes to wheat quality breeding was also discussed.
出处
《安徽农业科学》
CAS
北大核心
2006年第18期4530-4531,4541,共3页
Journal of Anhui Agricultural Sciences
基金
教育部春晖计划项目(2004251008)
教育部留学回国人员科研启动基金
