摘要
目的从肿瘤坏死因子α(TNFα)基因-857位点多态性角度探讨三氯乙烯药疹样皮炎易感的遗传背景。方法采用TaqManMGB探针,建立TNFα-857C/T位点实时定量聚合酶链反应分型方法。用该方法对三氯乙烯药疹样皮炎组(47例)及对照组(53例)进行-857nt(C/T)位点分型,比较病例组与对照组之间各基因型及等位基因频率是否有差异。结果TNFα-857C/T多态的基因型及等位基因频率在三氯乙烯药疹样皮炎组及对照组差异无显著性(P>0.05)。结论TNFα-857C/T位点多态可能不是中国人群三氯乙烯药疹样皮炎的风险因子。
Objective To study the genetic background of susceptibility to medicamentosa-like dermatitis induced by TCE at the point of tumor necrosis factor α gene （TNF α） polymorphism at - 857 nt （C/T） position. Methods The real-time polymerase chain reaction genotyping at - 857 nt （C/T） position of TNFα was established with TaMman minor groove binding （MGB） probes, and the patients （47） and the controls （53） were then genotyped and the frequency of genotype or allele was compared between these two groups. Results Neither the frequency of genotype nor allele of TNFα - 857 C/T was significantly different between the patient and control groups （ P 〉 0. 05 ）. Conclusion The TNFα - 857 C/T polymorphism may not be a risk factor to medicamentosa-like dermatitis caused by TCE in Chinese.
出处
《中国职业医学》
CAS
北大核心
2006年第5期349-351,共3页
China Occupational Medicine
基金
国家"973"计划资助项目(编号:2002CB512904)
广东省医学科学技术研究基金资助项目(编号:2003A710)
深圳市科技计划项目(编号:200304157)
关键词
三氯乙烯药疹样皮炎
肿瘤坏死因子Α
单核苷酸多态性
Medicamentosa-like dermatitis caused by TCE
Tumour necrosis factor α
Single nucleotide polymorphisms
