摘要
目的研究血中甲胺磷的分析方法及血样灌流吸附后对毒物的定量清除率。方法采用HA230树脂吸附柱对中毒血样进行灌流吸附，将吸附后血样经乙酸乙酯萃取后，用火焰光度检测的毛细管气相色谱法测定其含量并计算清除率。结果分析方法的检出限为0．05ng，低、中、高三种浓度的加标回收率为94．3％～102．7％，相对标准偏差为4，38％～5．12％。灌流吸附后血中毒物的清除效率为60．6％～73．4％。结论用HA230树脂吸附剂进行血液的灌流吸附，能清除大部分毒物，迅速降低血中甲胺磷浓度。对抢救重度甲胺磷中毒的危重病人有重要价值。
Objective In order to study the Cleanup- rate of methamidophos in blood sample after hemopeffusion. Methods We put methamidophos standard solution into the health human blood, and take it through a HA230 resin hemoperfusion cartridge having the huge absorption capacity, and then the blood samples were extracted by acetic ether, at last the contents of methamidophos were determined by Capillary Gas Chromatography （GC）. Results The limit low line of methamidophos in mankind blood was 0.05ng. The recoveries of low, middle and high three kinds added were between 94.3 % and 102.7 %. The relative standard deviations （RSD） were between 4.38 % and 5.12 %. The Cleanup - rate is 60.6 % -7a. 4 %. Conclusion The poisons mostly were removed out from blood after poisons absorption through HA230 resin hemoperfusion and the concentration of methamidophos in blood was reduced rapidly.
出处
《济宁医学院学报》
2006年第3期1-3,共3页
Journal of Jining Medical University
基金
国家"十五"攻关课题资助项目(2001BA704B06)
