摘要
目的构建一个选择性感染噬菌体(selectively infective phage,SIP)载体。方法删去M13KO7gIII基因的第2个甘氨酸丰富区,并在此位置插入3个内切酶识别位点,用于外源基因的插入,构建成重组噬菌体基因组BP-M13KO7。将重组噬菌体BP-M13KO7的培养液上清液感染XL1-Blue,检测其感染背景。结果重组噬菌体BP-M13KO7培养液上清液不具有感染性,完全消除了背景。结论通过将M13KO7gIII基因的第2个甘氨酸丰富区置换成3个内切酶识别位点,构建成的重组噬菌体无感染性背景。
Objective To construct a non-background selectively infective phage （SIP） vector. Methods The second Gly-rich region in gⅢp of MI3KO7 was deleted, and replaced with a fragment containing 3 enzyme recognition sites for foreign genes. The infectious background of the resulting phage was examined by infecting culture supernatant of BP-M13KO7 with XL1-Blue. Results Infectivity background was not found. A non-background SIP was successfully constructed. Conclusion Selectively infective phage, constructed by displacing the second Gly-rich region in gⅢp of M13KO7 with fragment containing 3 enzyme recognition sites, is completely deleted with infectious background.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2006年第5期484-486,共3页
Immunological Journal
基金
国家自然科学基金资助项目(30271580)
