摘要
目的研究不同剂量GM-1(神经节苷脂)对离体大鼠神经干细胞增殖和分化的影响。方法自大鼠脑组织分离神经干细胞,培养于含有bFGF和/或B27的DMEM/F12培养液中,实验组培养液中分别加入33.5μg/L、3.35μg/L及0.335μg/LGM-1,用MTT比色分析法测定细胞增殖能力。用含体积分数3%血清的DMEM/F12培养液诱导细胞分化,每间隔6h于倒置显微镜下观察细胞分化情况。结果4d、8dMTT比色显示,GM-1(33.5μg/L)组与阳性对照组比较,P均>0.05,与阴性对照组比较,P均<0.05;GM-1(3.35μg/L)组、GM-1(0.335μg/L)组与阴性对照组比较,P均>0.05。分化研究发现,接种6h后,3个实验组与阴性对照组的细胞突起短且细小,而血清组神经求周边可见明显粗大的细胞突起,呈放射状向周边伸出;随着分化时间的延长,各组细胞突起均逐渐伸长,3个实验组分化能力低于对照组,与阴性对照组之间无显著差异。结论GM-1能够维持大鼠神经干细胞的原始状态,促进神经干细胞增殖,而对神经干细胞的分化作用不明显。
Objective To study the effects of different dose of GM - 1 on the growth and differentiation of neural stem cells. Methods The neural stem cells were obtained from mouse brains and cultured in serum - free medium - DMEM/F12 with bFGF and B27, different dosage of GM - 1 was added into the different experimental group, and the proliferation ability of the cells were measured by MTT methods. The cells were induced to differentiate in the medium - DMEM/F12 with 3% serum and observed through inverted phase- contrast microscope every 6 hours. Results According to test , the 4 d and 8 d MTT showed as follows： there was no significant difference lay in positive- control group and GM- 1 （33.5μg/L） group, however, the significant difference lay in GM- 1 （33.5μg/L）group and negative- control group, there was no significant difference between GM - 1（0.335μg/L）group,GM- 1 （3.35μg/L）group and negative-control group. The differential experiments showed that： the neurites of the three experiment groups and negative control group were shot and thin, while the neurites of the neuralsphere of（3% ）serum ＋ DMEM/F12 group stuck in a radial way after the neuralsphere being replanted in 24 - well plate coated with poly- lysine for six hours. The neurites of each group developed little by little with the time gone by, however, the neurites of the three experimental groups and the negative- control group were much less than those of positive -- control group. Conclusion GM - ] has ability to maintain the primary situation of neural stem cells and has effects on their growth, while there was no obvious influence on their differentiation.
出处
《医学信息（手术学分册）》
2006年第2期57-59,共3页
Medical Information Operations Sciences Fascicule
