摘要
目的探讨重组人生长激素(rhGH)对肝脏缺血再灌注损伤的保护作用及其机理。方法Pringle法建立100只肝脏缺血再灌注损伤动物模型,rhGH组于建立模型前7d每天给予rhGH针剂皮下注射(0.2U/100g体重),对照组等量生理盐水注射,观察其血清ALT、TNF-α和IL-1α的变化,检测肝细胞中丙二醛(MDA)、肝脏能荷(energycharge,EC)的变化及核转录因子-κB(NF-κB)和细胞间黏附分子-1(ICAM-1)mRNA的表达,电镜观察肝脏的超微结构变化。结果rhGH组ALT、TNF-α、IL-1α和MDA在各个时间点的水平均明显低于对照组(P<0.05),rhGH组EC水平明显高于对照组(P<0.05);rhGH组肝细胞核因子NF-κB表达及ICAM-1mRNA的表达水平均明显低于对照组(P<0.05)。对照组电镜下可见肝窦内皮细胞破坏,肝细胞线粒体结构改变,rhGH组肝细胞超微结构明显改善。结论rhGH可能通过下调NF-κB的表达,进一步抑制了细胞因子(TNF-α、IL-1α)和ICAM-1致炎因子mRNA的表达,减少MDA的生成,从而减轻了这些因子对肝脏的损伤。
Objective To investigate the protective role of recombinant human growth hormone （rhGH）in ischemic reperfusion injury of rat liver and its mechanism. Methods One hundred Male rats were randomly divided into two groups： the rhGH group and the control group. In the rhGH group, rhGH were injected （0. 2U/100g weight） to rats seven days before the ischemic reperfusion injury, and in the control group, normal saline was injected instead. Serum levels of ALT, TNF α and IL-1α were tested. Hepatic tissue was sectioned for to detect the level of EC and MDA, the expression of NF-kB and ICAM-1 mRNA on SEC. Ultrastructural characteristics histopatho logical characteristics were determined also. Results Serum levels of ALT, TNF-α, IL-1α and the contents of MDA in the control group were significantly higher than those in the rhGH group （P〈0. 05）. Comparied with control group, rhGH also decreased NF-kB activation, and reduced the expression of ICAM 1 mRNA of SEC in the liver cells （P〈0.05）. Electronic microscopic revealed that the hepatic sinusoidal endothelial cells and the hepatocellular mitochondria were injured in the control group. Pretreatment with the rhGH was able to significantly improved the pathological changes. Conclusion rhGH might confer the protection to ischemic reperfusion injury of rat liver through reducing the expression of NF-kB to down-regulate cytokine （IL-1α,TNF-α）, MDA and inhibition the ex pression of ICAM-1 mRNA.
出处
《中国普外基础与临床杂志》
CAS
2006年第5期555-559,共5页
Chinese Journal of Bases and Clinics In General Surgery
关键词
重组人生长激素
大鼠
缺血再灌注损伤
丙二醛
核转录因子-KB
细胞间黏附分子-1
Recombinant human growth hormone Rat Ischemic reperfusion injury Malondial- dehyde Nuclear factor kappa B Intercellular adhesion molecule-1
