摘要
[目的]研究胶原支架(CS)对磷酸钙骨水泥(CPC)的力学及其在体内成骨的影响。[方法]试验分CPC/CS及CPC两组,三点弯曲试验测试材料的强度和弹性模量;组织学观察材料植入兔股骨22及54周的成骨状况。[结果]CPC/CS比CPC的弯曲强度、韧性强度分别提高了64.2%、3 933.3%,弹性模量降低了45.7%;组织学显示22周CPC/CS内的胶原支架已完全被新骨替代,CPC只在边缘有少量成骨及材料降解而内部无成骨;54周CPC/CS已大部分降解孔化,孔内充满大量新骨及髓样组织,而CPC边缘区的成骨及材料降解虽比22周时明显,但其内部仍未见成骨。[结论]胶原支架既可改善CPC的力学性能,又能促进新骨长入CPC/CS复合材料内部,因此,CPC/胶原支架是较好的骨缺损修复材料。
[ Objective] To examine whether collagen scaffold（CS） would strengthen calcium phosphate cement （CPC） in vitro, and to investigate whether CPC enhanced with CS would accelerate the osteogenesis for repairing bone defect in rabbits. [ Method] A standard 3-piont flexural test was used to fracture the set specimens and to measure the flexural strength, work-offracture（WOF） and elastic modulus of CPC and cpclcs. Both cements were implanted into cylinder cavities drilled in 20 rabbit femoral condyles. After 22 and 54 weeks of implantation, histological observations were performed. [ Result] The CS had significant effects on composite of mechanical properties. The CPC/CS had a flexural strength 64.2% higher, WOF 3 933.3% higher, and elastic modulus 45.7% lower than the CPC without CS. At 22 weeks, histological study showed collagen fibers of the CPC/ CS composite were entirely resorbed and new bone was formed instead. With unreinforced CPC, there were a little bone formation and biodegradation of cement on the margin but not in the interior of CPC. At 54 weeks, plentiful growing bone and bone marrowlike tissue fully filled in pores created by biodegradation of CPC/CS. Although bone formation and biodegradation of cement were more obvious on the margin of CPC cylinder at 54 weeks than 22 weeks, new bone did not present in the interior of CPC yet. [ Conclusion] Collagen scaffold not only reinforces CPC, but also accelerates bone formation in the interior of CPC/CS composite. Therefore, CPC/CS could be novel biomaterial as an endosteal implant.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2006年第18期1410-1412,共3页
Orthopedic Journal of China
基金
北京自然科学基金资助项目(2982007)
关键词
磷酸钙骨水泥
胶原支架
骨形成
calcium phosphate cement
collagen scaffold
osteogenesis
