摘要
目的探讨组胺对大鼠脑片皮质内血管的调控作用和机制。方法免疫组化方法检测H1和H2受体在大鼠脑皮质内血管的表达。采用鉴别干涉对比显微镜观察大鼠脑片额叶皮质血管,实时检测和记录组胺引起的血管内径变化。为观察组胺对脑皮质内血管的收缩作用是否与组胺H1、H2受体有关,用H1受体拮抗剂苯海拉明和H2受体拮抗剂西咪替丁预处理脑片,再观察组胺的作用。结果H1受体和H2受体在脑皮质血管壁呈免疫反应阳性。组胺产生浓度依赖性血管收缩,血管收缩率从(1.48±0.67)%到(32.91±7.91)%。除在高浓度(30、100μmol/L)之间没有显著性差异(P>0.05)外,各浓度间都有显著性差异(P<0.01)。苯海拉明预处理脑片,组胺没有引起血管变化。用西咪替丁预处理脑片,组胺仍引起血管收缩,各浓度组间存在显著性差异(P<0.05)。与单纯加入组胺相比,在10和30μmol/L浓度有显著性差异(P<0.05)。结论组胺可引起脑皮质内血管的收缩,其机制是通过H1受体介导。
Objective To investigate histamine-induced changes of the intracortical vessels in the cortical slice of rat brain. Methods Immunohistochemistry was employed to detect the expression of H1 and H2 receptors in the intracortical blood vessels of rats. Histamine-induced constriction of the intracortical blood vessels of the brain slices was observed with differential interference contrast microscope. Measurements of the luminal diameter were made on-line during the course of the experiment and confirmed off-line from the stored images. In order to observe whether histamine H1 and H2 receptors affected histamine-induced constriction, the intracortical blood vessels in the brain slices were pre-treated with H1 receptor antagonist diphenhydramine and H2 receptor antagonist cimetidine. Results Expression of ill and H2 receptors was detected in the intracortical blood vessels of the rat brain. Histamine （1-100μmol/L） induced a concentration-dependent constriction from （1.48±0.67）% to （32.91 ±7.91）%. The reactions to each histamine concentration were significantly （P〈0.01） different from each other, with the exception of the highest histamine concentrations （30 and 100 μmol/L） when maximal constriction due to histamine were observed （P〉0.05）. With pre-treatment of the slice with 10 μmol/L diphenhydramine, application of histamine did not elicit constriction. Pre-treatment of the slice with 10 μmol/L cimetidine did not completely inhibit but somehow significantly weakened vascular constriction in response to histamine treatment at 10 and 30 μmol/L （P〈0.05）. Conclusion Histamine can induce constriction of the intracortical blood vessels, which is mediated by H1 receptor.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2006年第9期1284-1287,共4页
Journal of Southern Medical University
基金
国家自然科学基金(30300109)~~
关键词
组胺
肺皮质血管
组胺受体
histamine
intracortical blood vessels
histamine receptors
