摘要
目的探讨广西百色地区壮族人群白细胞介素-1受体拮抗剂(IL-1Ra)基因第2号外显子T+8006 C位点多态性分布。方法采用聚合酶链反应和限制片段长度多态性(PCR-RFLP)方法检测了165例百色地区壮族健康人群IL-1Ra(+8006)位点多态性,并结合文献进行了不同种族间的分析比较。结果百色地区壮族健康人群基因型以TT型最为常见,其次为TC型,CC型较为少见,分布频率依次为0.863、0.148、0.006;其等位基因以T型最为常见,其次为C型。与美国、德国、日本等国家人群相比,该位点多态性均存在显著性差异(P<0.01),与国内湖北地区汉族人群相比无显著性差异(P>0.05)。结论IL-1Ra基因第2号外显子+8006位点存在T/C多态性,其在不同种族间的分布可能存在着明显的差异。
Objective To study the distribution of the second exon region ＋ 8006T/C polymorphism of interleukin-1Ra gene in the chinese Zhuang population of Bose in Guangxi province. Methods The second exon region ＋ 8006T/C polymorphism of interleukin-1Ra gene was detected by ploymerase chain reaction-restrictlon fragment length polymorphism technique in healthy Zhuangs（ n = 165 ）. Results The frequencies of TT , TC and CC genotypes were found to be 0. 863,0. 148 and 0.006 in Zhuangs . There was no significant difference in the frequency distribution of interleukin- 1Ra genotype between Bose Zhuangs and Hubei Hans （ P 〉 0.05 ） , but the polymorphism of IL - 1Ra（ ＋ 8006 ） in Zhuangs was significantly different compared with American, Germany and Japanese populations （ P 〈 0.01 ） . Conclusion There is a single nucleotide polymorphism（SNP） in the exon 2 of IL-1Ra at position ＋ 8006 in Chinese Zhuang population of Bose, whose distributions are probably significantly different in different ethic populations.
出处
《解剖科学进展》
CAS
2006年第3期199-201,共3页
Progress of Anatomical Sciences
基金
广西自然科学基金资助项目(桂科自0499004)
右江民族医学院课题(右医院字200486)
