摘要
目的探讨地塞米松对海水淹溺性肺损伤兔的肺泡钠水转运系统及肺水肿的影响。方法新西兰兔随机分成对照组(CG)和地塞米松治疗组(BG)。CG兔(n=12)的气管内灌注4 ml/kg海水,DG兔(n=12)在CG的基础上静注1 mg/kg地塞米松。肺水肿用血管外肺水含量指数(EVLWI)监测。给药3 h收集肺组织标本,分别用RT-PCR和Western印迹检测肺组织中钠离子通道-α亚单位mRNA(α-ENaC mRNA)和钠钾泵-α1亚单位(NKA-α1)蛋白的丰度以及水解法检测NKA活性。结果DG的EVLWI显著的低于CG[(0．508±0．089)vs．(0．648±0．102),P＜0．05],而α-ENaC mRNA和NKA-α1蛋白的丰度均显著的高于相应的CG(P＜0．05,P＜0．05),NKA活性也显著的高于CG(P＜0．05)。结论地塞米松可增加肺泡钠水转运系统的表达或活性,并减轻海水灌注肺损伤兔的肺水肿。
Objective To investigate the effects of dexamethasone on absorption of lung edema in rabbits with seawater drowning- induced acute lung injury. Methods Seawater （4 ml/kg body weight） was instilled into the lower trachea of ventilated and anesthetized rabbits. These rabbits were assigned randomly to receive intravenous injection of 1 mg/ kg body weight of dexamethasone （dexamethasone group, DG） or 2 ml of normal saline （eontrol group, CG） . Lung edenma as measured by extravascular lung water index （EVLW1） using a gravimetric method. Three hours after treatment, epithelial Na^± channel subunit-α （α- ENaC） mRNA and Na^±/K^± - adenosine triphosphatase subunit-α1 （NKA -α1 ） protein abundances in lung tissues were respectively measured by reverse transcriptase- polymerase chain reaction and Western blotting, and NKA activity was measured by monitoring the release of inorganic phosphate （Pi） from adenosine triphosphate （ATP） . Results The DG＇s EVLWI was significantly lower than the CG＇ s [ （0.508 ± 0.089） vs. （0.648 ± 0. 102）, P 〈 0.05）], but the DG＇s NKA activity, α - ENaC mRNA and NKA -α1 protein abundances were significantly higher than the CG＇s, correspondingly （ P 〈 0.05）. Conclusions With up - regulation of the NKA activity and expressions of α - ENaC and NKA -α1, dexamethasone treatment could promote the absorption of lung edema in rabbits with seawater drowing - induced acute lung injury.
出处
《中华急诊医学杂志》
CAS
CSCD
2006年第9期801-804,共4页
Chinese Journal of Emergency Medicine
基金
中国人民解放军总后医药卫生课题(2003)
关键词
急性肺损伤
肺水肿
淹溺
海水
地塞米松
Acute lung injury
Lung edema
Drowning, Seawater
Dexamethasone
