摘要
平滑肌细胞增殖并形成泡沫细胞是动脉粥样硬化的中晚期病变特点之一。本实验研究了巨噬细胞对平滑肌细胞清道夫受体活性及肌源性泡沫细胞形成的影响。用细胞酶联免疫吸附法测定平滑肌细胞对氧化型低密度脂蛋白的结合和摄入量以显示清道夫受体活性，另测定细胞内胆固醇含量以显示脂质沉积。结果发现：巨噬细胞条件培养液可促进平滑肌细胞清道失受体的表达和平滑肌细胞内胆固醇积聚；巨噬细胞的含脂碎片也能促使平滑肌细胞内胆固醇的增加。结果提示，巨噬细胞可分泌某些因子促进平滑肌细胞的资道夫受体表达及其胞内的脂质积聚，这两种细胞的相互作用是动脉粥样硬化发展的一个重要机制。
The smooth muscle cell-derived foam cell formation is one of the characteristics at the intermediate and late stages of atherosclerosis, In this paper we studied the effect of macrophages on the formation of smooth muscle cell-derived foam cells,The volumes of oxidized low density lipoprotein binding and uptake were measured with cell enzyme linked immunosorbent assay method to show the scavenger receptor activities,The contents of intracellular cholesterol were also measured.The results indicat ed that macrophage conditioned medium could induce the smooth muscle cells ( SMC ) scavenger receptor expression and its intracellular cholesterol accumulation,and that increase the SMC intracellular cholesterol contents, This supported that macrophages could release some factors to enhance the SMC scavenger receptor expression and the intracellular lipid accumulation, and that the interaction between SMC and macrophages may play an important role in the development of atherosclerosis.
出处
《中国动脉硬化杂志》
CAS
CSCD
1996年第4期260-263,共4页
Chinese Journal of Arteriosclerosis
关键词
平滑肌细胞
泡沫细胞
巨噬细胞
动脉粥样硬化
Smooth muscle cells
Foam cells
Macrophages
Scavenger receptors
Atheroscle rosis
