摘要
AIM： To investigate the implication of angiogenin （ANG） in the neovascularizaton and growth of human gastric carcinoma （HGC）. METHODS： ANG mRNA expression in HGC specimens obtained by surgical resection from patients with HGC were examined by RT-PCR. ANG, Ki-67, VEGF protein expression and microvessel density （MVD） in HGC specimens were detected by immunohistochemistry. RESULTS： RT-PCR showed significantly higher ANG mRNA expression （0.482 ± 0.094） in HGC tissues than in the surrounding nontumorous tissues （0.276 ±0.019, P = 0.03）. MVD within tumorous tissues increased significantly with ANG mRNA expression （r = 0.380, P = 0.001） and ANG protein expression （P 〈 0.01）. The ANG expression levels of cancer tissues were positively correlated with VEGF （P 〈 0.01） and the proliferation index of cancer cells （P 〈 0.01）. CONCLUSION： ANG is one of the neovascularization factors of HGC. ANG may work in coordination with VEGF, and promote the proliferation of HGC cells.
瞄准：在人的胃的癌(HGC ) 的 neovascularizaton 和生长调查 angiogenin (ANG ) 的含意。方法：在外科的切除术与 HGC 从病人获得的 HGC 标本的 ANG mRNA 表示被 RT-PCR 检验。在 HGC 标本的 ANG， Ki-67， VEGF 蛋白质表示和微容器密度(MVD ) 被免疫组织化学检测。结果：RT-PCR 显示出显著地更高的 ANG mRNA 表示(0.482 +/- 0.094 ) 在 HGC 纸巾比在包围非肿胀的纸巾(0.276 +/- 0.019， P = 0.03 ) 。在肿胀的纸巾以内的 MVD 与 ANG mRNA 表示显著地增加了(r = 0.380， P = 0.001 ) 并且 ANG 蛋白质表示(P < 0.01 ) 。癌症纸巾的 ANG 表示层次断然与 VEGF 被相关(P < 0.01 ) 并且癌症房间的增长索引(P < 0.01 ) 。结论：ANG 是 HGC 的 neovascularization 因素之一。ANG 可以与 VEGF 在协作工作，并且支持 HGC 房间的增长。
基金
Supported by the Natural Science Foundation of Fujian Province,No.C0110013Science and Technology KeyProgram Foundation of Fujian Province, No. 2002Y003
