摘要
目的探讨地塞米松对体外循环(CPB)诱发犬心、肺、脾及外周血单核细胞(PBMC)Toll样受体-4(TLR-4)mRNA表达的影响。方法健康家犬10只,体重18～24kg,雌雄不拘,随机分为对照(C)组和地塞米松(D)组,每组5只。麻醉诱导前即刻D组静脉注射地塞米松1 mg／kg,C组给予等量生理盐水,麻醉诱导后建立CPB,测定CPB前、CPB 1 h、CPB 2 h、停CPB后30 min、停CPB后2 h时心、肺、脾、PBMC中TLR-4、TNF-α、热休克蛋白(HSP-70)mRNA表达及血浆一氧化氮(NO)、丙二醛(MDA)浓度。结果CPB可诱发心、肺、脾及PBMC中TLR-4、HSP-70、TNF-αmRNA表达及血浆NO、MDA浓度增加;地塞米松预先给药可减弱CPB诱发的上述改变(脾除外)(P<0．05)。结论地塞米松预先给药可减弱CPB诱发犬的全身炎性反应,与下调TLR-4 mRNA表达有关。
Objective To investigate the effect of dexamethasone （DXM） on the regulation of Toll-like receptor-4 （TLR-4） mRNA expression in the heart, lung, spleen and peripheral blood mononuclear cells （PBMCs） during and after cardiopulmonary bypass （CPB）. Methods Ten healthy dogs of both sexes weighing 18-24 kg were randomized into 2 groups （ n = 5 each） ： dexamethasone （DXM） group received DXM 1 mg·kg^-1 i.v. before induction of anesthesia and control group received normal saline. Anesthesia was induced with intravenous pentobarbital, fentanyl, paneuronium and maintained with fentanyl, γ-OH and pancuronium. The animals were mechanically ventilated after tracheal intubation. Blood samples and specimens from right auricle, right lung and spleen were obtained and PBMCs were isolated for determination of plasma NO, MDA concentrations and TNF-α, HSP-70 and TLR-4 mRNA expression in the tissues and cells before CPB （T1 ,baseline） at 1 and 2 h of CPB （T2, T3 ） and 30 min and 2 h after discontinuation of CPB （ T4 , T5 ） . Results The two groups were comparable with respect to F/M ratio, body weight, Hct and CPB time. CPB caused significant increase in plasma NO and MDA concentrations and TLR-4, HSP-70 and TNF-α mRNA expression in heart, lung, spleen and PBMCs which were significantly attenuated by DXM pretreatment except spleen. Conclusion Dexamethasone pretreatment can attenuate the systemic imflammatory response to CPB by down-regulating the TLR-4 mRNA expression.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2006年第7期591-594,共4页
Chinese Journal of Anesthesiology
关键词
地塞米松
心肺转流术
受体
细胞表面
RNA
信使
Dexamethsone
Cardiopulmonary bypass
Receptors, cell surface
RNA, messenger
