摘要
将免疫酶斑点试验(DIBA)与布病常规血清学诊断方法相比较,用流行病学方法和成本—效益经济分析对DIBA进行了综合评价。结果初步表明DIBA在布病实验室诊断及对职业人群的流行病学调查均具有较高的实用价值,其灵敏度90％,特异度96％,准确度93％,可用度86％;且DIBA与ELISA 具有较高的相关性(r=0.9581P<0.01);并可采用微量耳垂全血替代肘静脉血清作为检测布病的样品;初步探讨了非特异性背景着色及内源性酶等因素对DIBA的影响。评价表明DIBA适用于基层单位使用和流行病学现场调查。
Dot immunobinding assay (DIBA) was compared with routine serological tests in the diagnosis of brucellosis and general evaluation of the method was made by epidemiological and cost-effective analyses. Results showed that DIBA, with a sensitivity of 90%, specificity of 96%, accuracy of 93% and usability of 86%, was of great practical value in laboratorial diagnosis and epidemiological survey of occupational population. It was also demonstrated that DIBA and ELISA were closely correlated (r = 0.9581, P< 0.01). In addition, whole blood of vena auricularsis (B.A) was used in this trial instead of venous serum of cutitus (S.C) as specimens in the test. And factors such as non-specific background color and intrinsic enzyme which could affect DIBA were discussed. It was thus concluded that DIBA was of great help for primary health units in the diagnosis of brucellosis and in the field survey of epidemiological studies.
出处
《地方病通报》
1990年第3期67-73,共7页
Endemic Diseases Bulletin
关键词
布鲁氏菌病
免疫酶斑点
斑点试验
Brucellosis
Serologic diagnosis
Dot immunobinding assay (DIBA)
Epidemiological evaluation
