摘要
本文以克山病病区粮喂养大白鼠为对照,以病区粮加钼或/和硒作实验观察,对大鼠心肌和肝线粒体膜脂流动性、呼吸控制率(RCR)及氧化磷酸化效率(ADP/O比值)作了研究。实验结果表明,病区粮饲料中加入适量的硒(0.23ppm)可使上述指标明显改善;饲料中加入较大量的钼(40ppm)可以起到与硒相仿的作用,而加入少量钼(2ppm)几无作用。硒钼同时加入饲料,仅在肝线粒体RCR见到明显协同作用,其余指标与单独加硒时基本相当。此外,在该两元素合用组观察到肝线粒体膜脂流动性明显低于其他处理组(加钼_1组除外)而与对照相当,但其RCR及ADP/O比值明显高于对照。对此膜脂流动性与RCR及ADP/O比值不相一致的原因尚不清楚。 作者认为本实验中大量钼的作用应属药理作用,缺硒仍为克山病的主要发病因素,本实验结果不支持缺钼病因。
An attempt was made to find out the primary factor of Keshan disease (KSD) by comparing the effects of molybdenum and selenium on mitochondria of the heart and the liver of rats fet on diet from a KSD affected area. The membrane fluidity, respiratory control ratio(RCR) and oxidative phosphorylation ratio (ADP/O) of the mitochondria were measured.
Results showed that supplementary Se (0.05ppm) in diet could improve the three indices mentioned above (P<0.01 or P<0.05); the effect of the large dose of Mo (40 ppm) in diet was similar to that of Se; the smaller dose of Mo (2ppm), however, had hardly any function (P>0.05). Generally, the effects of selenium are more powerful than those of Mo; the functions of combination of both Se and Mo are similar to those of Se alone. In addition, in the group of combination of Se and Mo (Se 0.23ppm, Mo 2ppm), a separate phenomenon thatthe fluidity of hepatic mitochodria is low while its RCR and ADP/O ratio is high , was observed. The cause of the phenomenon is unknown.
It may be concluded that 1. the effect of the large dose of Mo on mitochondria should be attributed to pharmacologic function; 2. Se deficiency in vivo is still a primary factor of KSD. These data failed to support the Mo-deficiency of KSD.
出处
《地方病通报》
1990年第2期13-19,共7页
Endemic Diseases Bulletin
关键词
钼
硒
克山病
心肌
肝
线粒体
Molybdenum(Mo)
Selenium(Se)
Membrane fluidity
RCR
Mitochondrion
ADP / O ratio
Myocardium
Liver
Rat
