摘要
目的探讨肝抗原自身抗体在自身免疫性肝病患者血清中的阳性率。方法155例患者分为三组：自身免疫性肝病组45例,包括自身免疫性肝炎(AIH)15例、原发性胆汁性肝硬化(PBC)20例、原发性硬化性胆管炎(PSC)10例；病毒性肝炎组50例；不明原因肝损伤组30例。分别用间接免疫荧光法、免疫印迹法检测肝抗原(SAL/LP、LKM-1、LC-1、LG-1)自身抗体。结果抗SLP/LP抗体在AIH患者血清中阳性率为46．4%,明显高于LKM-1(13．3%)、LC-1(0%)及AMA-M2(13．3%)抗体,并且在病毒性肝炎患者血清中呈阴性反应。抗AMA-M2抗体在PBC患者血清中阳性率达95．0%。不明原因肝损伤组患者中有6．6%的AMA- M2抗体阳性。结论抗SLA/LP抗体对AIH具有特异性,肝抗原自身抗体的检测将有助于自身免疫性肝病患者的诊断及治疗。
Objective To study the significance of anti-antibody of liver antigens in the diagnosis of autoimmune hepatitis disease. Methods Patients were divided into three groups according the diseases：autoimmune hepatic disease 45 eases including autoimmune hepatitis（AIH） 15 cases, primary biliary cirrhosis（PBC） 20 cases, and primary sclerotic cholangitis（PSC） 10 cases;Various virus hepatitis 50 cases;Liver damage of unknown cause 30 cases. Auto-antibody of liver antigens SLA/LP, LKM-1, LC-1 ,and AMA-M2 were identified by indirect immunofluorescence （IIF） assay and immunoblot assay. Results The positive rates of anti-SLA/LP（46.6% ） was significantly higher than those of anti-LKM-1 （ 13.3 % ）, anti-LC-1 （0 % ）, and anti-AMA-M2 （ 13.3 % ） in patients with AIR while these four antibodies were negative in patients with virus hepatitis. The positive rates of anti-AMA-M2 in patients of PBC and unknown liver damage were 95.0 % and 6.6 %, respectively. Conclusion Anti-SLA/LP is a new specific serum marker in diagnosis of AIR. The auto-antibody detection of liver antigens will be helpful to the diagnosis and therapy of autoimmune hepatic disease.
出处
《中国基层医药》
CAS
2006年第8期1304-1306,共3页
Chinese Journal of Primary Medicine and Pharmacy
关键词
肝炎
自身免疫性
免疫印迹法
荧光抗体技术
间接
Hepatitis, autoimmune
Immunoblot assay
Fluorescent antibody technique,indirect
