摘要
应用标准玻璃微电极技术，研究哇巴因诱发豚鼠心室乳头肌的延迟后除极（ＤＡＤ）和触发电活动（ＴＡ）的变化规律及特点；探讨普罗帕酮和维拉帕米对ＤＡＤ和ＴＡ的影响。结果表明：①心室肌细胞在高浓度Ｃａ２＋条件下加哇巴因灌流时可以出现ＤＡＤ及ＴＡ，ＴＡ的诱发率为７７．８％（７／９）。②心室肌细胞诱发的ＤＡＤ幅度（ＤＡＤ－Ａｍｐ）、ＤＡＤ偶联间期（ＤＡＤ－Ｃ）和ＤＡＤ升支上升速率（ＤＡＤ－ｄｖ／ｄｔ）呈明显的频率依赖性，即刺激频率愈快，ＤＡＤ－Ａｍｐ愈高、ＤＡＤ－ｄｖ／ｄｔ愈快，ＤＡＤ－Ｃ则愈短。③上述两种抗心律失常药物均通过降低ＤＡＤ－Ａｍｐ、减慢ＤＡＤ－ｄｖ／ｄｔ和延长ＤＡＤ－Ｃ而发挥其抗触发性心律失常作用；两者对哇巴因诱发的ＤＡＤ和ＴＡ的抑制作用无显著性差异，在普罗帕酮或维拉帕米的作用下，哇巴因对ＴＡ的诱发率分别为３３．３％（３／９）和２２．２％（２／９），与哇巴因的诱发率（高钙条件下，７７．８％）比较Ｐ＜０．０１。
The effect of ouabain on delayed afterdepolarization (DAD) and triggered activity (TA) in guinea pig papillary muscles was studied by standard microelectrode method and the effects of propafenone and verapamil on DAD and TA were also studied by the same way.Results showed:(1)TA could be induced by the elevation of the concertration of calcium or ouabain and it occurred repeatedly in the same circumstance.Incidence of TA was 77.8%(7/9) .(2)The amplitude (DAD Amp),coupling interval (DAD C),and the mean rate of rise (DAD dv/dt ) of DAD were frequency dependent.(3)Propafenone and verapamil could decrease DAD Amp,slow down DAD dv/dt ,and prolong DAD C.The difference of the effects of inhibition on DAD and TA induced by ouabain between propafenone and verapamil was not significant.Incidence of TA were respectively about 33.3%(3/9) and 22.2%(2/9) on effect of propafenone and verapamil.
出处
《中国心脏起搏与心电生理杂志》
1996年第4期210-213,共4页
Chinese Journal of Cardiac Pacing and Electrophysiology
关键词
普罗帕酮
维拉帕米
细胞电生理学
触发电活动
Propafenone Verapamil Guinea pig papillary muscle Delayed afterdepolarization Triggered activity Guinea pig
