摘要
将正反义classⅠpatatin基因导入马铃薯品种甘农薯2号中,有2个转正义基因株系试管块茎的可溶性蛋白含量和LAH活性与对照相比有不同程度的增加,有3个转反义基因株系的可溶性蛋白质含量下降,并且所有转反义基因植株的LAH活性都不同程度地降低。试管块茎的诱导结果表明,有1个转正义基因株系的结薯株率和单株结薯数比其对照明显增加,有2个转反义基因株系的结薯株率和单株结薯数比对照明显减少,说明该classⅠpatatin基因参与了马铃薯试管块茎的形成及其调控。
Sense and antisense class Ⅰ patatin gene were introduced into potato cuhivar Gannongshu 2. Two sense lines had a marked higher soluble protein content and LAH activity than the control. Three antisense lines showed a reduction in soluble protein content, but all of antisense lines displayed a decrease in LAH activity. Induction of microtuber demonstrated that one sense line had a significant increase while two antisense lines had a significant reduction in percentage of plantlets formed tubers and number of tubers per plantlet. The results suggested that the class Ⅰ patatin gene is involved in regulating potato tuber formation.
出处
《作物学报》
CAS
CSCD
北大核心
2006年第9期1406-1409,共4页
Acta Agronomica Sinica
基金
国家自然科学基金资助项目(39970464)
