摘要
采用Plackett-Burman设计对影响柳小皮伞(Marasmiussalicicola)AS5.166发酵胞外多糖培养基的组成成分进行了筛选,所选取的10个相关因素为:葡萄糖、蔗糖、酵母膏、蛋白胨、K2HPO4、KH2PO4、CaCl2、FeSO4、MgSO4、VB1。结果表明,影响柳小皮伞(Marasmiussalicicola)AS5.166胞外多糖含量的主要因素为葡萄糖、蔗糖、酵母膏、KH2PO4、MgSO4和FeSO4。
In previous study, it was indicated that the production of extracellular polysaccharide by Marasmius salicicola AS 5.166 were strongly affected by many factors during fermentation. In this study, the gradual step analysis method was used to regulate the medium composition and enhance extracellular polysaccharide production. Firstly, Plackett-Burman design was employed to evaluate the effects of the ten components in the medium, including glucose, sucrose, yeast extract, peptone, KH2PO4, K2HPO4, FeSO4, CaCl2, MgSO4 and VB1. In further analysis, it was confirmed that glucose, sucrose, yeast extract, KH2PO4, MgSO4 and FeSO4 had strong effect on the production of extracellular polysaccharide by M. salicicola AS 5.166.
出处
《中国酿造》
CAS
北大核心
2006年第9期24-26,共3页
China Brewing
基金
蚌埠学院院级科研项目(2004bbxy106)
