摘要
目的:了解慢性特发性血小板减少性紫癜(CITP)患者骨髓、血液中B淋巴细胞协同刺激因子CD40的表达情况,从而进一步了解CITP的免疫学发病机制。方法:采用流式细胞术检测CITP患者的骨髓及末梢血的淋巴细胞协同刺激因子CD40的表达,并用ELISA方法检测血液中的PAIgG。结果:CITP组末梢血B淋巴细胞CD40表达率(13.81%±2.98%)明显高于正常对照组(6.31%±1.24%),两者比较差异有统计学意义(P<0.01);CITP组骨髓B淋巴细胞CD40表达率(23.65%±3.25%)也明显高于正常对照组(8.32%±2.34%),两者比较差异亦有统计学意义(P<0.01);CITP患者的外周血B淋巴细胞CD40表达率与PAIgG水平两者间呈显著的正相关(r=0.92,P<0.05)。结论:CD40在CITP患者淋巴细胞的表达异常增高,可能是CITP发病的免疫学机制之一。
Objective：To investigate the effect of costimulatory factors CD40 on the pathogenesis of chronic idi opathic thrombocytopenic purpura （CITP）. Method： Expression of CD40 on B lymphocyte of CITP patients＇ bone marrow and peripheral blood was measured by flow cytometry, and serum PAIgG of CITP patients was measured by ELISA. Result：CD40 expression in B lymphocyte of CITP patients＂ peripheral blood was obviously higher than that of the control group （13.81%±2.98% vs 6.31%± 1.24%, P〈0.01） ; CD40 expression in B lymphocyte of CITP patients＇ bone marrow was obviously higher than that of the control group （23.65%±3.25%vs 8.32%±2.34%）; CD40 expression in B lymphocyte of CITP patients＇ peripheral blood had positive correlation with PAIgG （ r=0.92, P〈0.05）. Conclusion; CD40 expression in B lymphocyte of CITP patients＇ bone marrow and peripheral blood significantly increased, this may be one of the immune pathogenesis of CITP.
出处
《临床血液学杂志》
CAS
2006年第5期291-293,共3页
Journal of Clinical Hematology
