摘要
目的探讨乙型肝炎病毒前S1抗原与乙型肝炎病毒DNA(HBV DNA)、乙型肝炎e抗原(HBeAg)、抗HBe(Anti-HBe)的相关性。方法对450例乙肝患者血清进行酶免法(EIA)检测前S1抗原、微粒子酶免分析法(MEIA)检测HBeAg、定量PCR内标法检测HBV-DNA。同时对用MEIA检测乙型肝炎病毒血清标志物均阴性的72例正常健康人血清也进行前S1抗原检测。结果450例HBV-DNA阳性的乙肝患者中,HBeAg阳性率为76．8％,前S1抗原的阳性率为61．5％,其中:前S1抗原的阳性率在HBeAg阳性患者中为61．6％、在抗-HBe阳性患者中为61．5％。HBV-DNA阳性情况下HBeAg阳性与HBeAg阴性患者的前S1抗原阳性率,两组经x2检验,P>0．05无明显差异。结论前S1抗原、HBeAg与HBV DNA符合率较高,前S1抗原与HBeAg无明确相关。对无条件检测HBV DNA而HBeAg阴性的血清,检测前S1抗原,可起到提示病毒是否复制的补充作用,如与其他乙型肝炎病毒血清标志物同时检测,对临床会更有意义。
Objective To better understand the duplication of hepatitis B virus （HBV） and suggest clinical diagnosis and treatment via comparison of the correlation of HBV-DNA, HBeAg with HBV Pre-S1 antigen. Methods For the 450 HBsAg positive patients, measure HBV Pre-S1 antigen with ELISA and HBV-DNA by using COBAS AMPLICOR HBV MONITORTM reagent and COBAS AMPLICOR quantitative PCR instrument; proceed with MEIA （microparticle enzyme immunoassay analysis） to make use of the fully automatic enzyme Immunoassay analysis instrument made by Abbott Axsym of the U. S. to detect HBeAg and Anti-HBe. Results Before treatment to the 450 patients of Hepatitis B virus infection, the positive rate of HBeAg was 76. 8% and HBV PreS1 antigen was 61.5% in the HBV-DNA positive patients. HBV Pre-S1 antigen in the 450 HBV-DNA positive and HBeAg positive patients was 59. 54% positive; in HBV-DNA positive and Anti-HBe positive was 61.54% positive. Conclusions HBV Pre-S1 antigen accompany with HBV-DNA, It was not influenced by HBeAg. For the results of HBV-DNA positive but HBeAg negtive, it is clinically significant to detect PreS1 antigen in Hepatitis B immunological examination.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2006年第8期714-716,共3页
Chinese Journal of Laboratory Medicine
