摘要
为了制备孔隙呈球形、孔隙间相互连通的高孔隙率三维细胞支架,研究了一种新型水溶性球形致孔剂及性质。探讨了间歇离心湿度粘技术与水溶性球形致孔剂相结合,制备聚乳酸(PDLLA)三维多孔细胞支架,获得了高度为5·0mm±0·5mm,直径为8·3±0·4mm的三维多孔支架。实验结果表明,致孔剂颗粒呈球形、水溶性良好,在三维支架中无残留;并且支架的孔隙结构均匀、孔隙率高(94·68%±0·52%)、孔隙呈球形、孔隙间相互完全连通,孔隙的大小可以人为控制。由此表明,在组织工程细胞支架制备中,这类水溶性球形致孔剂具有广泛的潜在应用价值。
For the purpose of three-dimensional scaffolds fabrication with completely interconnected spherical pores for tissue engineering application, a novel water dissoluble spherical porogen was studied in this paper. The porogen was applied to build porous three-dimensional scaffolds. Poly （D, L-lactic acid） scaffolds of 5.0mm±0.5mm in thickness, 8.3mm±0.4mm in diameter with porosity of 94.68%±0.52% were formed. It was showed that the scaffolds with spherical pores and highly interconnected openings were obtained. The pore sizes and diameters of the openings were controllable by varying the particulate sizes. The porogen was easily leaching out of scaffolds, there was no porogen remains were detected in scaffolds. Those results suggested that the porogen has potential application in tissue engineering.
出处
《中国生物医学工程学报》
CAS
CSCD
北大核心
2006年第2期197-201,共5页
Chinese Journal of Biomedical Engineering
基金
上海市博士后基金
(863计划)组织工程化骨构建的关键技术与产品开发(2002AA205011)
关键词
三维细胞支架
溶液浇注/颗粒沥析
组织工程
致孔剂
three-dimensional cells scaffolds
solvent casting/particulate leaching
tissue engineering
porogen
