摘要
目的建立豚鼠卡那霉素耳毒性聋模型,观察银杏叶提取物对卡那霉素耳毒性聋的早期干预作用。方法将豚鼠随机分成正常对照组(不做任何处理)、卡那霉素组(连续肌肉注射卡那霉素7天)、银杏叶提取物干预组(肌注卡那霉素的同时,于不同时间同时注射银杏叶提取物金钠多)。测定各组动物听性脑干反应(ABR),通过比较各组豚鼠波Ⅲ阈值、100dB SPL声刺激时波Ⅲ潜伏期和峰值差异,评价银杏叶提取物对早期卡那霉素耳毒性的干预作用。结果卡那霉素组ABR波Ⅲ阈值高于正常对照组,差异有统计学意义(P<0.05),说明本实验中卡那霉素成功致聋;卡那霉素组和银杏叶提取物干预各组之间的波Ⅲ阈值差异无统计学意义(P>0.05)。结论卡那霉素耳毒性聋的早期使用银杏叶提取物可能没有任何保护和治疗作用,是否加重耳毒性作用还有待进一步研究。
Objective To establish an experimental kanamycin- induced deafness model for the study of EGB761 as the early intervention for kanamycin - induced deafness in guinea pig. Methods The guinea pigs were injected with kanamycin. At the same time, the EGB761 - intervene groups were set up. After the experiment , the auditory function was measured with ABR. Results The animal model of kanamycin- induced deafness was successfirUy established. The auditory function of the EGB761 - intervene group was not worse or better than that of kanamycin group. Conclusion EGB761 shows neither protection nor treatment effect as the early intervention to kanamycin - induced deafness, on the contrary, this can expose guinea pigs to greater kanamycin ototoxicity.
出处
《听力学及言语疾病杂志》
CAS
CSCD
2006年第5期354-356,共3页
Journal of Audiology and Speech Pathology
