摘要
采用微量液体培养法,对8例正常人及16例急性髓细胞白血病(AML)病人化疗前骨髓单个核细胞(BMC)进行体外培养1周,动态观察BMC的氚-胸腺嘧啶核苷酸(~3H-TdR)掺入率,发现AML患者化疗前BMC最初3天内增殖较活跃,且多有增殖峰出现,部分病例在第6天前后出现第2个增殖峰.但各病例间个体差异较大.动态观察亦表明AML的BMC在体外培养的前5天内较正常人的BMC增殖明显活跃,差别具有非常显著性意义(P<O.01).动态观察的结果表明化疗前AML的BMC增殖动力学与临床疗效有关,表现为化疗前BMC增殖活跃,则临床缓解可能性大,反之,如一直处于低增殖状况则临床上不易缓解.
Using a microliquid culture method. We observed sequetially the prolif-erative kinetics of bone marrow mononuclear cell (BMC) which corporated ^(3) H-TdR from one to seven days. It included 8 normal adults, 16 patients with acute myel-ogenous leukemia (AMD. The patients with AML had higher proliferative activity during the initial 3 days, and leukemia cells had a peak on proliferative curves. By sequential observations, we found that the patients with AML had higher proliferative activity than normal adults, and the difference wes significant during the initial > 5 days in vitro ( P <0. 01). we found that the proliferative actirity was related to the prognosis, ie,if the patients with AML had higher proliferative activity in vitro, they would be more likely to attain remission, and vice versa.
出处
《临床血液学杂志》
CAS
1996年第4期157-159,共3页
Journal of Clinical Hematology
