摘要
分别以RBCL基因和PRSV-CP基因、PRSV-RP基因为内源基因和目标基因,设计了3对特异性引物,对转基因番木瓜进行了SYBRGreen实时荧光PCR检测.结果表明,RBCL基因引物对所有供试样品成功扩增,Ct值为15～16,PRSV-CP引物对转PRSV-CP基因番木瓜样品成功扩增,Ct值为20～25,PRSV-RP引物对转PRSV-RP基因番木瓜样品成功扩增,Ct值为21～22.运用熔解曲线进行产物分析,验证了试验结果的特异性和准确性.
This thesis studied on the detection of genetically modified papaya by real-time fluorescent SYBR Green PCR using the ribulose bisphosphate carboxylase/oxygenase large subunit as the endogenesis reference gene and the papaya ringspot virus coat protein, replicating protein gene as the target gene. The results showed that RBCL primers can successfully amplify all the experimental samples, Ct value was 15-16; PRSV-CP primers can successfully amplify the experimental samples which contained PRSV-CP gene, Ct value was 20-25; PRSV-RP primers can successfully amplify the experimental samples which contained PRSV-RP gene, Ct value was 21-22. The amplified products were analyzed by the melting sequence allis, it also oroved that the experiment results were differential and veracious.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第4期371-374,共4页
Journal of Hunan Agricultural University(Natural Sciences)
基金
深圳出入境检验检疫局项目(SZK03-2002)
