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从发病仔猪呼吸道检测出猪腺病毒 被引量:5

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摘要 根据猪腺病毒六邻体保守区域设计两对引物,用Nested-PCR的方法从送检的发病仔猪的呼吸道分泌物中扩增出两个核酸片段。通过基因克隆、序列测定和序列比较,发现这两个核酸片段与猪腺病毒5型和3型的相应片段同源性最高,分别为97.4%和95.3%。证实发病仔猪呼吸道存在猪腺病毒的感染。为下一步猪腺病毒的分离及猪腺病毒表达载体的构建打下基础。
出处 《广西畜牧兽医》 2006年第5期204-206,共3页 Guangxi Journal of Animal Husbandry & Veterinary Medicine
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参考文献8

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同被引文献54

  • 1李厚伟,魏战勇,郭显坡,陈红英,李金磊,韩志涛,崔保安.实时定量PCR探讨猪细小病毒自然感染猪体内病毒的分布情况[J].西北农林科技大学学报(自然科学版),2010,38(11):1-6. 被引量:3
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