摘要
目的:探讨新型脑血管治疗药物马来酸桂哌齐特注射液(克林澳)对创伤性脑损伤后病理组织学、活化部分凝血活酶时间(APTT)及线粒体酶活性的影响。方法:Sprague-Dawley大鼠随机分3组:假手术组(n=10);液压(压力1.8～2.2atm)脑损伤对照组(n=20):创伤后30min和24h尾静脉注射生理盐水0.5ml;液压脑损伤药物组(n=20):创伤后30min和24h尾静脉注射克林澳3.0mg/kg。于损伤后72h检测创伤侧皮层、海马组织病理损伤,同时检测不同时间点出凝血系列指标以及代谢关键酶α-酮戊二酸脱氢酶(KGDHC)的活性变化。结果:脑损伤后72h血液呈高凝状态,APTT显著低于对照组(P<0.05),α-KGDHC活性显著降低(P<0.05),应用克林澳后出凝血指标与对照组差异无显著性,而α-KGDHC活性显著增高,神经元损伤显著减轻。药物组的皮层和海马神经元计数较对照组分别增加51%和35%(P<0.05)。结论:脑损伤后早期应用克林澳不改变血液学特性,但对代谢酶有显著影响,减轻神经元损伤。
Objective To investigate the effect of a neotype cerebrovascular regulation drug cinepazide maleate injection in traumatic brain injury （TBI）. Methods Fifty male Sprague-Dawley rats were randomly divided into three groups： sham-operation group（n=10）, moderate fluid-percussion TBI group（n=20, saline 0.5 ml at 30 min and 24 h of post-injury） and drug infusion group （n=20, cinepazide maleate 3.0 mg/kg at 30 min and 24 h of post-injury）. The animals were killed at 72 h of post-injury, and the brain samples （the cerebral cortex, hippocampus and thalamus） were harvested for pathological assay, α-ketoglutaric dehydrogenase （α-KGDHC） activity assay and APTT assay were detected at different time point. Results The survival neurons of the cortex and hippocampus CA1 decreased to 55% and 53% respectively at 72 h after TBI. In comparison with the moderate fluid-percussion TBI group, the neurons of the cortex, hippocampus and thalamus in the drug infusion group decreased 51%, 36% and 21%, respectively （P〈0.05）. The neurological outcome was also better in the drug infusion group. Conclusions Early injection of cinepazide maleate may improve the neurological outcome and ameliorate the pathological lesions after brain injury.
出处
《诊断学理论与实践》
2006年第4期319-322,共4页
Journal of Diagnostics Concepts & Practice
基金
国家自然科学基金资助
基金编号:30571908
关键词
马来酸桂哌齐特
脑损伤
活化部分凝血活酶时间
α-酮戊二酸脱氢酶
Cinepazide maleate
Traumatic brain injury, Activated partial thromboplastin time
α-ketoglutaric dehydrogenase
