摘要
[目的]观察大段活性人工骨对大动物骨缺损的修复效果,了解材料的降解性。[方法]以快速成形技术制备犬用PLLA.cTCP大段人工骨载体材料,按3 mg/块材料的标准复合rhBMP-2制备犬用大段活性人工骨。以犬桡骨2.0 cm骨缺损为实验模型,将大段活性人工骨(实验组)和单纯载体材料(对照组)植入骨缺损,通过影像学、组织学、生物力学检查评价骨缺损的修复效果,通过图像分析仪分析材料的降解情况。[结果]影像学检查表明,实验组术后12周骨痂与断端完全连接,术后24周骨痂塑形良好。对照组术后24周无骨痂生长,缺损未修复。组织学检查证明,实验组术后12周骨痂外层形成板层骨,中央形成小梁骨及骨髓组织,材料部分吸收;术后24周,骨痂板层骨致密,小梁骨减少,骨髓组织增多,材料进一步降解。对照组术后12周纤维组织将材料包裹并长入其中,材料部分降解;术后24周材料被纤维组织分割包裹,材料进一步降解。术后12周实验组和对照组的降解率分别为43.2%和35.7%,术后24周58.4%和45.4%。生物力学检测证明,术后24周实验组桡骨的抗弯强度超过正常骨的强度。[结论]PLLA.cTCP大段活性人工骨对大动物骨缺损有良好的修复效果,材料的降解性还需要改善。
[ Objective] To evaluate the effect of massive bioactive bone substitute in repairing large animal＇s bone defect and to know its degrading rate. [ Method] The massive Polylevolactic acid · collage calcium phosphate （PLLA· cTCP） carrieres by rapid forming technology was making, and then compounding rhBMP-2 and carrieres in a ratio of 3mg rhBMP-2 to one carrier was compounded to prepare the massive bioactive bone substitutes for dog＇s bone defect. Then the massive bone substitutes were implanted into 2. Ocm dogs＇s radius defects in the experiment group, and the massive carriers were implanted into in the control group. The repairing effect was evaluated by radiography, histology and biomechanics, and the degrading rate of the substitues was calculated in an image analysis apparatus. [ Result] Radiographically, in the experiment group, the defects were connected by callus in all dogs in 12 weeks postoperatively; in 24 weeks, the callus rebuilt well. But in the control group, there was no callus formed in 24 weeks postoperatively, and the defects were not repaired. Histologically, in 12 weeks postoperatively, the outer layer of the callus in the experiment groups was lamellar bone and the center were trabecular bone, myeloid tissue and partial degrading carrier; in 24 weeks, the lamellar bone was more compact, trabecular bone decreased, myeloid tissue increased, and the carrier degraded more. In the control group, in 12 weeks postoperatively, the fibrous tissue wrapped and infiltrated into carrier, at the same time, part of the carrier degraded; in 24 weeks, the carrier was divided up by fibrous tissue and degraded more. The degrading rate of the carder in 12 weeks in the experiment group was 43.2% , in the control group was 35.7%, in 24 weeks 58.4% and 45.4%. Biomechanics, in 24 weeks after postoperation, the radius strength in the experimentgroup was superior to that in the normal bone. [ Conclusion] The massive bioactive bone substitutes have satisfactory repairing effect on the radius bone defects of the large animal, but its degrading rate needs improving.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2006年第17期1336-1338,i0003,共4页
Orthopedic Journal of China
基金
国家高技术研究发展计划(863计划)资助项目(No.715-009-0160)
