摘要
目的：探讨外源性三磷酸腺苷（ATP）对大鼠移植胰腺的作用和机制．方法：建立SD大鼠同种异位全胰十二指肠移植模型，供体胰腺分别用肝素平衡盐液或肝素平衡盐添加ATP液进行低温（0—4℃）灌注并保存60min后行移植手术，检测再灌注0，1，6，12h时血糖、脂肪酶、淀粉酶含量，同时检查胰腺组织病理学变化，ATP含量及凋亡、胀亡细胞百分数．结果：ATP组胰腺组织结构损伤明显轻于对照组，ATP6，12h组血糖、血清淀粉酶、脂肪酶含量明显低于同时间点对照组（血糖，脂肪酶，P〈0．05；淀粉酶，P〈0．01），ATP0，1h组胰腺组织ATP含量明显高于同时间点对照组（P〈0．01），ATP组各时间点胀亡细胞百分数明显小于同时间点对照组（0，1，6h，P〈0．01；12h，P〈0．05），ATP含量与胀亡细胞百分数明显负相关（r=-0．756，P〈0．01），与凋亡细胞百分数无明显相关．结论：外源性ATP对移植胰腺有保护作用，减少胰腺组织细胞胀亡的发生可能是其重要的保护机制．
AIM： To study the protective effect of exogenous adenosine triphosphate （ATP） on transplanted pancreas in rats and its mechanism. METHODS： The allogeneic male SD rat models of heterotopic whole pancreaticoduodenal transplantation were established. The isolated pancreas of rats was perfused and stored with calparine balanced solution in which ATP was added as an additive agent in ATP group. No ATP was added to calparine balanced solution in control group. The organs were stored at 0 -4℃ for 60 min in both groups. Blood sugar,plasma concentrations of amylase and lipase were examined and histological observation was performed at 0, 1, 6 and 12 h after reperfusion. The concentration of ATP in pancreatic tissue was determined. The percentages of normal, apoptotic and oncotic cells were measured. RESULTS： Light microscopic observation showed that histomor- phological changes of pancreas in ATP group were much milder than those in control group. The blood sugar concent, plasma concentrations of amylase and lipase were significantly lower in ATP group than those in control group at 6, 12 h after reperfusion （ blood sugar, lipase, P 〈 0.05 ; amylase, P 〈 0.01 ）. Tissue concentration of ATP in ATP group was higher than that in control group at 0, 1 h after reperfusion （ P 〈 0.01 ）. The percentage of oncotic cells was significantly lower in ATP group than that in control group at all time points after reperfusion （0, 1,6 h, P 〈 0.01; 12 h, P〈0.05）. Tissue concentration of ATP had a significant negative correlation with the percentage of oncotic cells （r = - 0. 756, P 〈 0.01 ）, but no evident correlation with the percentage of apoptotic cells. CONCLUSION： Exogenous ATP has a protective effect on transplanted pancreas in rats. The mechanism of the protective effect maybe lies in that ATP can decrease the percentage of oncotic cells.
出处
《第四军医大学学报》
北大核心
2006年第16期1477-1479,共3页
Journal of the Fourth Military Medical University
关键词
胰十二指肠移植
三磷酸腺苷
细胞凋亡
细胞胀亡
panereatieoduodenal transplantation
adenosine triphosphate
apoptosis
oncosis
