摘要
为了探索雌、孕激素受体（ＥＲ、ＰＲ）的检测方法和阳性结果判断标准的规范化，应用ＥＲ、ＰＲ单克隆抗体（ＭｏＡｂ）免疫组化Ｓ－Ｐ法检测乳腺癌石蜡切片中ＥＲ、ＰＲ的表达。结果显示：２００例ＥＲ及ＰＲ的阳性率分别为７３．５％及６５．５％。用单克隆抗体可直接显示ＥＲ、ＰＲ，结果可靠、敏感性高、重复性好，且与目前国际通用方法接轨。此技术成功的关键在于抗原修复。通过２３例的比较，确定了最佳修复时间和温度。并探讨了阳性结果的评判标准。我们认为用阳性细胞百分比法较阳性细胞强度指数法更为合理、可行。
Theexpressionofestrogenreceptor(ER)andprogesteronereceptor(PR)in200breastcancerspecimenswereimvestigatedbymonoclonalantibodywithS-Pstainingtoevaluateandstandardizethecriteriaforpositivestaining.TheresultsrevealedthattheincidenceofERandPRwere73.5%and65.5%respectively.TheauthorsconcludedthattheimmunostainingcoulddemonstrateinsituERandPR,theresultswerestable,highlysensitive,consistentandcomparablewiththewelestablishedandrecognizedtechniqueshomeandabroad.Theauthorsfurtherpointedoutthatthecrucialpointofthestainingisantigenretrievalandreportedtheoptimaltemperatureandthetimeofsodiumcitratebuffertreatment.Inthisreport,thecriteriaforpositivestainingwerediscussedintensively.Theobservationsindicatedthatthepercentageofthepositivecelsismorereliable,representativeandpracticalthanthatofpositivestainingintensityindex.
出处
《中华病理学杂志》
CSCD
北大核心
1996年第6期329-331,共3页
Chinese Journal of Pathology
关键词
乳腺癌
雌激素
孕激素
受体
免疫组织化学
BreastneoplasmsReceptors
steroidAntigenicmodulationImmunohis-tochemistry
