摘要
应用固相抗原筛选法，建立了人抗ＨＢｓＡｇ单克隆抗体组合文库。以液相ＨＢｓＡｇ包被酶标板，对抗ＨＢｓＡｇ血清阳性供者文库经多次筛选、扩增，建立起针对ＨＢｓＡｇ的组合抗体子文库。选取１２个克隆进行抗体片段表达研究，并对该抗体进行特异性酶联免疫测定，其中５株为抗－ＨＢｓ阳性，应用组合文库制备人源单克隆抗体是─种简便、实用的方法。
Con the methods of antigen based selections,one human monoclonal antibody fragment library to hepatitis B surface antigeus(HBsAg)was generated. Liquid HBsAg were coated on the ELISA plate and one combinatorial antibody library that was from a HBsAg-positive donor was panned with the HBsAg coated wells,The antigen specific phages in the library were selected and enriched to form a HBsAg-specific library,Choosing 12 clones for Fab expression and anti-HBsAg specificity study,Fab supernatants from 5 clones show anti-HBs positive in enzyme linked immunoassay. Results showed that application of combinatorial library to prepare specific Fabs against viral diseases is a attractive and hopeful fields.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1996年第5期299-302,共4页
Chinese Journal of Immunology
关键词
单克隆抗体
组合抗本文库
乙型肝炎
Human monoclonal antibody
Combinatorial library
Type B hepatitis
