摘要
以黄鳝为实验动物,研究了不同剂量的三氧化二砷对黄鳝的毒理学效应。采用鱼类致突变实验、鱼类红、白血球计数等方法,测定外周血白细胞数、红细胞微核和核异常以及肝脏过氧化氢酶(CAT)活性。结果发现:在低剂量范围内,随着三氧化二砷剂量的增加红细胞的微核率及核异常率逐渐增加、CAT活性升高,白细胞数则下降;至0.50mg/kg剂量时红细胞的微核率、核异常率及CAT活性均达最大值,而白细胞数达最低值;随着剂量的进一步增加红细胞的微核率、核异常率以及CAT活性均反而下降,白细胞数则上升。结果表明一定剂量三氧化二砷能明显影响黄鳝外周血细胞及肝脏CAT活性。
To study the toxicological effects of the different dosages of As203 on Monopterus albus. The methods of the mutagenesis test and red and white blood cells counting in fish were used to assay the white cell number, micronuclei and nuclear abnormity of red cell in peripheral blood erythrocytes, and CAT activity of liver in Monopterus albus. Results show that the rate of micronuclei and the rate of nuclear abnormity, and CAT activity gradually increase, white cell number gradually decrease, in rang of lower dosage. At 0.50 mg/kg they are respectively the highest and the lowest. With the dosage continuing to increase, the frequency of micronuclei and nuclear abnormity of red cell, and CAT activity decrease instead, white cell number gradually increase. The experiment enunciates that the some dosage of As2O3 can effect on erythrocytes and leucocyte of peripheral blood, and CAT activity of liver in Monopterus albus.
出处
《细胞生物学杂志》
CAS
CSCD
2006年第4期627-631,共5页
Chinese Journal of Cell Biology
基金
浙江省自然科学基金(No.M303606)
温州市科技局科研基金(No.S2002A015)
温州师范学院科研基金(No.2003Z20)~~
关键词
三氧化二砷
毒理学效应
微核率
核异常率
肝脏过氧化氢酶活性
rsenic trioxide
toxicological effects
micronucleus frequency
frequency of nuclear abnormity
CAT activity of liver
