摘要
目的探讨Egr-内皮抑素(Egr-Endostatin,Egr-Endo)基因联合放射治疗抑制裸鼠膀胱癌移植瘤生长的效应及作用机理。方法裸鼠皮下注射人膀胱癌T24细胞建立移植瘤模型,待肿瘤长径生长到约4 mm后随机分为4组(对照组、Egr-Endo组、5 Gy辐照组、Egr-Endo+5 Gy辐照组),每组6只。定期观察各组裸鼠移植瘤体积变化;瘤细胞移植45 d后(辐照后15d)处死各组荷瘤鼠,测量瘤重、检测NK细胞毒活性和腹腔巨噬细胞TNF-α分泌活性,免疫组化法检测肿瘤组织微血管密度(MVD)。结果瘤细胞移植45 d后(辐照后15d)Egr-Endo+5 Gy辐照组移植瘤体积、重量明显低于对照组、Egr-Endo组、5 Gy辐照组,差异有显著性;辐照后15 d Egr-Endo+5Gy辐照组NK细胞毒活性、腹腔巨噬细胞TNF-α分泌活性及肿瘤组织MVD均明显高于对照组,差异有显著性。结论Egr-Endo基因联合放射治疗对膀胱癌生长及血管生成有协同抑制作用。
Objective To explore the combined inhlbitory effects of Egr-Endostatin gene and radiotherapy on bladder transplantable tumor in nude mice and its possible mechanism. Methods Nude mice model was established by subcutaneouly injecting human bladder cancer T24 cell line, when tumor diameter arroved to 4 mm, 24 nude mice were averagely randomized into four groups （control group, Egr-Endo group, 5 Gy irradiation group, Egr-Endo＋5 Gy irradiation group）. The tumor volume was measured periodically, nude mice were killed 45 d after transplanled carcinoma cells （ 15 d after irradiation） to measure tumor weight and to detect cytotoxic activity of NK and TNF-αsecretion activity of peritoneal macrophage and to determine the intratumor microvessel density （MVD） with immunohistochemical method. Results The implantation tumor volume and weight in Egr-Endo ＋ 5 Gy irradiation group 15 d after irradiation were significantly lower than those in control group, Egr-Endo group and 5 Gy irradiation group.Ctotoxic activity of NK, TNF-α secretion activity of peritoneal macrophage and MVD in Egr-Endo ＋ 5 Gy irradiation group 15 d after irradiation were significantly higher than those in control group. Conclusions The combination of Egr-Endostatin gene with radioflaerapy can synergistically inhibit the bladder tumor growth and neovascularization on nude mice model.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2006年第2期225-227,共3页
Chinese Journal of Gerontology
