摘要
目的:观察心肌营养素-1(CT-1)在高糖高胰岛素诱导的肥大心肌细胞中的表达,以及吡格列酮对其表达的影响。方法:体外培养乳鼠心肌细胞,分组给药后,检测心肌细胞肥大的指标:心肌细胞的表面积(图像分析法)、蛋白含量(考马斯亮蓝法)、心房利钠因子(ANF)mRNA的表达(RT-PCR法);同时用半定量RT-PCR检测CT-1 mRNA的表达。结果:高糖高胰岛素组心肌细胞表面积、蛋白含量、ANFmRNA表达以及CT-1 mRNA水平均升高(P<0.01),而吡格列酮治疗组降低(P<0.05)。结论:高糖高胰岛素诱导的肥大心肌中CT-1表达上升,而吡格列酮能抑制其表达,推测CT-1可能参与了高糖高胰岛素所引起的心肌肥大的发生,而吡格列酮抑制心肌肥大的作用可能是通过抑制CT-1介导的。
Objective： To study the expression of cardiotrophin-1 （CT-1） in hypertrophic cardiac myocytes induced by high glucose and insulin, and the effect of pioglitazone on the expression of cardiotrophin-1 （CT-1）. Methods： The cardiac myocytes were cultured in vitro as a model. Cellular surface, protein content and atrial natriuretic factor mRNA were measured as the cellular hypearophic parameters. Meanwhile, the expression of cardiotrophin-1 mRNA was assayed with semiquantitative RT-PCR. Results： After treatment with high glucose and insulin, cellular surface, protein content and expression of atrial natriuretic factor mRNA increased significantly （ P 〈 0.01 ）. Pioglitazone inhibited all these above effects effectively （ P 〈 0.05）. Condusion： The results suggest that cardiotrophi-1 is involved in the development of myocardial hypertrophy induced by high glucose and insulin. And pioglitazone may inhibit myocardial hypertrophy by restraining the expression of cardiotrophin-1.
出处
《山东大学学报（医学版）》
CAS
北大核心
2006年第8期765-769,共5页
Journal of Shandong University:Health Sciences
