摘要
目的研究沥青烟致癌和致突变作用的机制。方法用沥青烟对小鼠进行不同剂量(55、165 mg/m^3)和不同时间(30、60 d)的染毒,观察小鼠肺组织病理形态改变,并用流式细胞仪进行小鼠肺组织细胞DNA含量的检测和细胞周期的分析。结果随着沥青烟染毒时间和剂量的增加,小鼠肺组织病变表现为不同程度的不典型增生和原位癌；同时小鼠肺组织细胞各周期指数发生变化,G_1期细胞数下降,S期阻滞,进入G_2/M期的细胞减少,细胞增殖指数(PI)增加,异倍体指数(DI)升高,与对照组比较,差异均有统计学意义(P＜0．05)。染毒相同时间时,55 mg/m^3和165mg/m^3剂量组的PI均高于对照组,165 mg/m^3剂量组的DI高于55mg/m^3剂量组,差异均有统计学意义(P＜0．05或P＜0．01)。相同染毒剂量下,60 d处理组的DI[(1．16±1．51)×10^(-2)、(1．20±2．30)×10^(-2)]均高于30 d处理组[(1．14±0．88)×10^(-2)、(1．16±1．47)×10^(-2)],差异有统计学意义(P＜0．05)。结论沥青烟所致小鼠肺组织产生的癌前病变可能与细胞周期的改变有关。
Objective To investigate the carcinogenic and mutagenic mechanism of bitumen fume. Methods The experimental mice were forced to inhale the bitumen fume at different exposure level（55 mg/m^3, 165 mg/m^3） and in different time（30 days,60 days）. The pathological changes of the lung tissue in mice were observed with H. E staining. The content of the DNA in the lung tissue of mice and the cell circles were determined with flow cytometry. Results The lesion of the lung tissue in mice comprised the atypical hyperplasia of different levels and the carcinoma in situ with the increase of the containment time and dosage;the cycle index was changed： the number of the G 1 phase cells was decreased, the S phase was retarded, the cells entering the G 2/S phase were decreased,the cell proliferation index（PI） was increased and the heteroploid DNA index（DI） was increased（ P 〈 0. 05） .The cell index in the 55 mg/m^3 group and the 165 mg/m^3 group was higher than that in the control group when the containment time was same. The heteroploid DNA index（DI） in the 55 mg/m^3 group was significantly higher than that in the 165 mg/m^3 group（P 〈 0.05 or P 〈0.01）.When the containment dosage was same,the DI in the 60 days treatment group（ 1.16 ± 1.51 × 10^-2, 1.20 ± 2.3 × 10^-2 ） was all significantly higher than those in the 30 daystreatmentgroup（1.14±8.8×10^-2,1.16±1.47×10^-2）（P〈0.05）. Conclusion The precancerous lesion in the lung tissue of the mice induced by the bitumen fume may be related with the changes of the cell cycle.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2006年第7期406-408,共3页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
甘肃省科学技术厅科学事业费资助项目(QS041-C33-27)
关键词
烃类
流式细胞术
肺
细胞周期
Hydrocarbons
Flow cytometry
Lung
Cell cycle
