摘要
目的:研究麦冬皂苷体内代谢产物薯蓣皂苷元含量测定方法。方法:采用HPLC-MS分析对大鼠口服麦冬皂苷肠溶微球的代谢产物之一薯蓣皂苷元进行体内过程的监测。结果:HPLC-MS法的最低检测浓度为50 ng/mL,萃取回收率大于65%,方法回收率大于93%,日内日间精密度均小于10%,线性关系较好,空白无干扰,峰形良好。结论:所建立的薯蓣皂苷元HPLC-MS定量法符合分析的要求,为中药皂苷类成分的生物利用度研究提供有效的手段和依据。
AIM： To determine the low content of Ophiopogon japonicus saponin metabolite in vivo. METHODS： HPLC-MS method of determining rat＇ s metabolite diosgenin in vivo was established after single-dose oral Ophiopogon japonicus saponin enteric microsphere. RESULTS ： The detection limit was about 50 ng/mL, Linearity, precision of intra and inter-day and reproducibility of the method were good. CONCLUSION ： This method accords with the analysis requirement. It can give an effective measure and foundation for studying TCM saponin bioavailability.
出处
《中成药》
CAS
CSCD
北大核心
2006年第8期1178-1181,共4页
Chinese Traditional Patent Medicine
