摘要
目的探讨肺减容术(LVRS)对肺气肿兔膈肌骨架蛋白信使核糖核酸(mRNA)表达的影响。方法40只家兔随机分为正常对照组、肺气肿组、单纯开胸组和LVRS组,每组10只。正常对照组气管内滴入生理盐水,余3组采用烟熏加气管内滴入0.4%木瓜蛋白酶0.5m l/kg诱发肺气肿,建立肺气肿模型,其中肺气肿组不作手术干预;单纯开胸组仅行胸骨正中切口进入双侧胸膜腔,不行LVRS;LVRS组胸骨正中开胸后行双侧LVRS。采用逆转录-聚合酶链反应(RT-PCR)法检测膈肌组织中肌联蛋白(titin)和伴肌动蛋白(nebu lin)mRNA的表达。结果与正常对照组比较,肺气肿组和单纯开胸组膈肌组织肌联蛋白mRNA和伴肌动蛋白mRNA表达均显著降低(P<0.01),LVRS组亦降低(P<0.05),但高于肺气肿组和单纯开胸组(P<0.05)。结论LVRS能够使肺气肿兔膈肌组织中肌联蛋白和伴肌动蛋白得到明显恢复,这可能是术后膈肌功能得以恢复的分子基础。
Objective To investigate the effect of lung volume reduction surgery （LVRS） on messenger RNA expression levels of cytoskeletal proteins in diaphragmatic muscle tissues of emphysematous rabbits. Methods A total of 40 rabbits were randomly divided into 4 groups （10 rabbits in each group） ：normal control group, emphysema group, sham operation group and LVRS group. Rabbits in control group were intratracheally administered with 0.9% normal sodium, but those in other groups were intratracheally administered with 0.4% papain at the dose of 0.5 ml/kg and inhaled cigarette smoke to induce emphysema model. Then, rabbits in emphysema group were fed routinely, however, after median sternotomy , bilateral LVRS was performed in LVRS group but not in sham operation group. The mRNA expression levels of titin and nebulin in the diaphragmatic muscles of rabbits in each group were detected by reverse transcription-polymerase chain reaction（RT-PCR）. Results Compared with control group, the mRNA expression levels of titin and nebulin in the rabbit diaphragm of emphysema groups and sham operation group decreased significantly （P〈0.01 ）, so did those in LVRS group （P〈0.05）. But it increased significantly in LVRS group compared with emphysema group and sham operation group （P〈0.05）. Conclusion LVRS can increase the mRNA expression levels of titin and nebulin in diaphragmatic muscle tissues of emphysematous rabbits, which may be the associated mechanisms at the molecular level in restoring the functions of the emphysematous diaphragm by LVRS.
出处
《中国胸心血管外科临床杂志》
CAS
2006年第4期251-254,共4页
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
基金
重庆市科技攻关项目(20033301)
第三军医大学科研基金(2001)~~
关键词
肺减容术
肺气肿
骨架蛋白
信使核糖核酸
Lung volume reduction surgery
Pulmonary emphysema
Cytoskeletal proteins
Messenger RNA
