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他克莫司软膏对特应性皮炎皮损角质形成细胞Toll样受体2和4表达的影响 被引量：9

Effect of topical tacrolimus ointment on expression of Toll-like receptors 2 and 4 in lesional atopic dermatitis skin
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摘要 目的:探讨Toll样受体(Toll-like receptor,TLR)2和TLR4在特应性皮炎(atop ic derm atitis,AD)发病机制中的意义及外用他克莫司软膏(商品名,普特彼)对TLR2和TLR4表达的影响。方法:采用免疫组织化学法检测9例中至重度AD患者急性发作期炎性皮损与正常对照皮肤角质形成细胞TLR2和TLR4的表达,以及外用他克莫司软膏单一疗法治疗AD 3周后TLR2和TLR4表达的变化。结果:免疫组化检测显示在正常皮肤基底层角质形成细胞胞膜散在表达TLR2和TLR4。TLR2和TLR4在AD急性发作期炎性皮损角质形成细胞胞膜及胞浆过度表达,以细胞膜表达为主,主要定位于基底层至颗粒层;他克莫司治疗3周后改善特应性皮炎皮损角质形成细胞的TLR2和TLR4表达,主要定位于基底或基底上层角质形成细胞膜。结论:正常角质形成细胞表达的TLR2和TLR4是构成皮肤天然免疫系统的组成部分。AD角质形成细胞诱导性过度表达TLR2和TLR4与AD皮肤天然免疫炎症反应有关,外用他克莫司可通过直接或间接作用抑制角质形成细胞的TLR2和TLR4诱导性表达,从而抑制与TLR细-胞核因子κB(NFκB)信号转导和调控途径有关的AD皮肤天然免疫炎症反应。 Objective ： To investgate the role of Toll-like atopic dermatitis（AD） and the effect of topical tacrolimus receptor（TLR） 2 and TLR4 in pathogenesis of ointment on expression of TLR2 and TLR4 in lesional AD skin. Methods： Immunohistochemistry was employed to study the expression of TLR2 and TLR4 in normal skin and lesional AD skin before and after using topical tacrolimus ointment. Results： The basal keratinocytes in normal skin constitutively expressed TLR2 and TLR4. In contrast , lesional epidermis from 9 patients with acute AD overexpressed TLR2 and TLR4 on the whole epidermis keratinocytes with membranous and cytoplasmic staining pattern. After using topical tacrolimus ointment for three weeks, TLR2 and TLR4 were expressed on basal and suprabasal keratinocytes with membranous and cytoplasmic staining pattern. Conclusion： These data suggest that TLR2 and TLR4 expressed by epidermal keratinocytes constitute part of the innate immune system of the skin, and increased TLR2 and TLR4 expression may be related to the skin innate immuno-inflammatory response in atopic dermatitis. Topical tacrolimus may directly or indirectly inhibit or downregulate TLR2 and TLR4 expression in KC and inhibit skin innate immuno-inflammatory response related to TLR-NFKB signal transduction and regulation in atopic dermatitis.
作者 谢志强 刘玲玲 扬高云 朱学骏
机构地区 北京大学第一医院皮肤性病科 清华大学第一医院皮肤科
出处 《北京大学学报（医学版）》 CAS CSCD 北大核心 2006年第4期420-423,共4页 Journal of Peking University:Health Sciences
基金 教育部教育振兴行动计划特殊专项("九八五"工程)资助~~
关键词 皮炎 特应性 他罗利姆 受体 细胞表面 Dermatitis, atopic Tacrolimus Receptors, cell surface
分类号 R758.23 [医药卫生—皮肤病学与性病学]
  • 相关文献

参考文献16

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  • 5谢志强,刘玲玲,窦侠,闻卫兢,王娣,朱学骏.特应性皮炎皮损角质形成细胞NF-κB表达及外用他克莫司对其的影响[J].北京大学学报（医学版）,2004,36(5):487-490. 被引量：9
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二级参考文献13

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共引文献8

同被引文献91

引证文献9

二级引证文献49

北京大学学报（医学版）
2006年 第4期

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