摘要
目的:观察大鼠在吸入不同浓度异氟烷后,肺表面活性蛋白-A(surfactant prote in-A,SP-A)的含量及其基因表达的改变。方法:雄性W istar大鼠32只随机分为对照组(Control),0.7%(体积分数)异氟烷组,1.5%异氟烷组和2.0%异氟烷组,每组8只。各组分别吸入40%(体积分数)O2和设定浓度的异氟烷8 h,对照组只吸入40%O28 h,然后迅速处死大鼠。取肺组织用透射电镜观察肺泡Ⅱ型上皮细胞结构变化。检测支气管肺泡灌洗液中和Ⅱ型上皮细胞中SP-A的含量以及肺组织中SP-AmRNA的表达。结果:吸入异氟烷后,1.5%异氟烷组和2.0%异氟烷组的肺泡Ⅱ型上皮细胞可见明显形态学改变。各吸入异氟烷组的支气管肺泡灌洗液中SP-A的含量均较对照组显著下降(Control组,437 112±25 654;0.7%异氟烷组,355 789±28 116;1.5%异氟烷组,238 554±31 531;2.0%异氟烷组,223 632±25 710;P<0.01);1.5%异氟烷组和2.0%异氟烷组较0.7%异氟烷组下降更明显(P<0.01)。免疫组化显示:1.5%异氟烷组与2.0%异氟烷组与对照组肺泡Ⅱ型上皮细胞中SP-A的含量明显下降,而0.7%异氟烷组较对照组无明显变化。吸入异氟烷的各组SP-A mRNA的表达均有降低趋势,1.5%异氟烷与2.0%异氟烷组与对照组比较差异有统计学意义(Control组,1.47±0.18;1.5%异氟烷组,1.19±0.13;2.0%异氟烷组,1.13±0.12;P<0.01)。结论:吸入1.5%或更高浓度的异氟烷8 h,可降低大鼠肺组织SP-A的基因表达和蛋白合成,并可能影响其分泌和再摄取。
Objective：To examine the effects of different concentrations of Isoflurane on the level of surfactant protein A（SP-A） and the expression of SP-A mRNA in the lung of rats. Methods： Thirty-two male Wistar rats were randomly divided into 4 groups： control group received 40% （ volume fraction） O2 inhalation （40%O2 ,n =8） ; 0.7% isoflurane group （n =8） ,1.5% isoflurane group （n =8）. They and 2.0% isoflurane group （ n = 8 ） . They were treated with 40% 02 with 0.7% , 1.5% and 2.0% isoflurane respectively. In each group, the rats inhaled experimental gas for 8 hours, and then were put to death immediately. Morphological changes of type Ⅱ pneumocytes were observed by transmission electron microscopy. Surfactant protein content in broncho-alveolar lavage fluid （BALF） was measured with Western Blotting . The level of intracellular SP-A was examined with immunohistochemistry （ IHC ）. To assess the relative levels of SP-A mRNA in lung tissue, reverse transcriptase-polymerase chain reaction （RT-PCR） was used with the co-amplification of the ＂housekeeping＂ gene GAPDH as internal control. Results： Morphological changes of type Ⅱ pneumocytes were distinct in 1.5% isoflurane and 2.0% isoflurane groups. Isoflurane, after three doses, significantly reduced the SP-A content in BALF. Control group, 437 112±25 654;0.7% Isoflurane group,355 789 ±28 116; 1.5% Isoflurane group,238 554±31 531; 2.0% Isoflurane group,223 632 ±25 710 （P〈0.01 ） ; Intracellular SP-A content of 1.5% and 2.0% isoflurane groups was less than that of the control, whereas 0.7% isoflurane groups did not show any significant difference from the control. RT-PCR indicated a reduced expression of SP-A mRNA in the lung after 1.5% and 2.0% isoflurane exposure. Control group, 1.47± 0.18 ; 1.5% Isoflurane group, 1.19 ± 0.13 ;2.0% Isoflurane group, 1.13 ± 0.12 （P〈0.01 ）. Conclusion ： These findings indicate that 1.5% or more than 1.5% Isoflurane exposure for eight hours could decrease the expression of the SP-A mRNA and synthesis of SP-A in rats, and maybe it could also affect the secretion and reuse.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2006年第4期402-406,共5页
Journal of Peking University:Health Sciences
关键词
异氟醚
肺表面活性剂
基因表达
Isoflurane
Pulmonary surfactants
Gene expression
