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产谷氨酰胺合成酶发酵条件的研究 被引量：1

Research on the Optimum Fermentation Conditions of Glutamine Synthetase
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摘要 谷氨酰胺合成酶是应用广泛的生物酶类.以LNU0165,LNU0066和LNU0254为实验出发菌株,对不同pH、温度条件下产谷氨酰胺合成酶的最佳发酵条件进行了研究,通过测定谷氨酰胺合成酶,并进行比较选择高产菌株,LNU0165产酶活最高,其GS酶的最适温度为60℃,最适pH为7.5左右. Glutamine Synthetase （GS） is a biological enzyme which has been used widely. It was studied on the optimum fermentation conditions for producing glutamine synthetase in different temperatures and pH is about LNU 0165, LNU 0066 and LNU 0254 as an experimantal bacteria. The results show that LNU 0165 is a high - producing strain by determining activity of GS. Optimum temperature is 60℃ and pH is about 7.5 for GS activity.
作者 魏杰 候萧 李辉 刘宏生
机构地区 辽宁大学生命科学系
出处 《辽宁大学学报（自然科学版）》 CAS 2006年第3期253-256,共4页 Journal of Liaoning University：Natural Sciences Edition
基金 辽宁省科技攻关项目(2004205004)
关键词 谷氨酰胺合成酶 节杆菌 最适条件 Glutamine Synthetase Arthrobacter optimum condition
分类号 TQ92 [轻工技术与工程—发酵工程]
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参考文献12

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同被引文献12

  • 1张晓昱,邓张双,朱长虹,吕艳娜,王宏勋.激光诱变选育高产PUFAs真菌菌株的研究[J].激光生物学报,2005,14(2):129-133. 被引量：9
  • 2杨世平,邱德全.米曲霉产中性蛋白酶的适宜条件[J].湛江海洋大学学报,2005,25(3):47-51. 被引量：20
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  • 9Larisa A Bespalova,Lyudmila P Antonyuk,Vladimir V.Ignatov.Azospirillum brasilense glutamine synthetase:influenceof the activating metal ions on the enzyme properties[J].BioMetals,1999,12:115-121.
  • 10Lyudmila P Antonyuk,Victoria E Smimova,Alexander A Kamnev,et al.Influence of divalent cations on the catalytic properties and secondarystructure of unadenylylated glutamine synthetase from Azospirillumbrasilense[J].BioMetals,2001,14:13-22.

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辽宁大学学报（自然科学版）
2006年 第3期

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