摘要
用火炬松（Ｐｉｎｕｓｔａｅｄａ）成熟合子胚诱导产生的胚性愈伤组织建立了胚性细胞悬浮系。研究了细胞密度、继代时间和ＡＢＡ浓度等对悬浮条件下胚性胚柄细胞团（ＥＳＭ：Ｅｍｂｒｙｏｎａｌｓｕｓｐｅｎｓｏｒｍａｓｓ）及体细胞胚（ＳＥ：Ｓｏｍａｔｉｃｅｍｂｒｙｏ）形成的影响。结果表明：当细胞密度为６×１０３个／ｍｌ、继代时间为２个月、ＡＢＡ浓度为５ｍｇ／Ｌ时，最有利于ＥＳＭ及ＳＥ发生。在悬浮培养条件下，还观察到了裂生多胚及子叶原基的分化。
Embryogenic cell suspension lines were established from embryogenic calli from mature zygotic embryos cultLlred of loblolly pine. Effects of different cell density,subcul-ture time,and ABA concentration on the formation of ESM (Embryonal suspensor mass ) and SE(Somatic embryo) were studied. The results show that optimum cultur conditions are cell density of 6 ×103, subculture time of 2 months, and ABA concentration of 5 mg/ L. The formation of cleaved polyembryony and the differentiation of primordial cotyle-dons were observed in the condition of liquid culture.
出处
《西北植物学报》
CAS
CSCD
北大核心
1996年第4期399-402,共4页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家"863"课题
关键词
火炬松
细胞悬浮培养
体细胞胚胎发生
Pinus taeda, cell suspension culture, somatic embryogenesis, cleaved polyembryony
