摘要
目的建立金刚藤片中薯蓣皂苷元含量的测定方法。方法采用高效液相色谱法(HPLC法),色谱柱为Diamonsil^(TM)(钻石)C_(18)柱(250mm×4．6mm,5μm),柱温为室温,流速为1．0mL/min；ELSD漂移管温度为60℃,空气流速为1．6L/min。结果薯蓣皂苷元进样量在0．1085～2．170μg范围内与峰面积有良好的线性关系,r=0．9998,平均回收率为98．2%,RSD为0．92%。结论HPLC法操作简单、快速,测定结果准确可靠,适用于金刚藤片的质量控制。
Objective To establish the HPLC method for diosgenin in Smilax china L. tablets. Methods HPLC method was employed. The column type was Diamonsil^TM C （250 mm×4.6 mm, 5μm）.The column temperature was room temperature. Flow rate was 1.0 mL/min. ELSD temperature was 60℃. Air flow rate was 1.6 L/min. Results Diosgenin showed a good linearity within the concentration range of 0. 108 5 - 2. 170 μg, the correlation coefficient was 0. 999 8. The average recovery rate was 98. 2%. RSD was 0. 92%. Conclusion The assay method is simple and fast, The results are precise and trusty, so this assay method can be used for the quality control in manufacture of Smilax China L. tablets.
出处
《中国药业》
CAS
2006年第13期40-41,共2页
China Pharmaceuticals
