摘要
目的探讨血清对氧磷脂酶-1活性与血浆氧化低密度脂蛋白在动脉粥样硬化性脑梗死发病机制中的作用。方法采用分光光度法和酶联免疫吸附法检测127例动脉硬化性脑梗死患者血清对氧磷脂酶-1活性,以及血浆氧化型低密度脂蛋白、血浆血管性假血友病因子、血浆α颗粒膜蛋白-140(GMP-140)及血清一氧化氮的变化,并对各项指标间的关系进行相关分析。另选择128例同期行体格检查或经头部CT检查无异常的轻度颅脑创伤患者作为对照。结果脑梗死组和对照组受试者血清对氧磷脂酶-1活性均在国人正常值范围。但与对照组比较,脑梗死组患者血清对氧磷脂酶-1活性明显降低(P<0.05),血浆氧化低密度脂蛋白水平明显升高(P<0.05);而且血浆血管性假血友病因子、血浆α颗粒膜蛋白-140等项指标亦均高于对照组(P<0.01),血清一氧化氮水平低于对照组(P<0.01)。相关分析表明,血清对氧磷脂酶-1活性分别与血浆血管性假血友病因子、血浆α颗粒膜蛋白-140及血浆氧化低密度脂蛋白水平呈负相关(r=0.423,0.383,0.339;均P<0.01),与血清一氧化氮水平呈正相关(r=0.205,P<0.01);而血浆氧化低密度脂蛋白与血清一氧化氮水平呈负相关(r=0.223,P<0.01),与血浆血管性假血友病因子和血浆α颗粒膜蛋白-140水平呈正相关(r=0.315,0.203;均P<0.01)。血清对氧磷脂酶-1活性与血清高密度脂蛋白-胆固醇、载脂蛋白B之间无相关性(P>0.05)。结论脑梗死组患者血清对氧磷脂酶-1活性的下降与其血浆氧化低密度脂蛋白水平增高有关,而血清对氧磷脂酶-1活性下降及血浆氧化低密度脂蛋白水平增高是脑梗死发病危险因素。
Objective To determine the role of serum paraoxonase-1 （PON-1） activity and plasma oxidative low-density lipoproteins （ox-LDL） in the pathogenesis of atherosclerotic cerebral infarction. Methods Serum PON-1 activity and ox-LDL were measured with ultraviolet spectroscopy, plasma ox-LDL, von Willebrand factor （vWF）, α-granular membrane protein-140 （GMP-140） were detected with ELISA and serum NO level was examined with nitrate reductase chromatometry, and analyzed their relationship. In corresponding period, selected 128 minimal craniocerebral wound patients without abnormal physical examination or encephalo-CT findings as controls. Results Serum PON-1 activity was normal in cerebral infarction group and control group, but it was significantly lower and ox-LDL level was significantly higher in cerebral infarction group than in control group （P〈0.05）. In comparison with control group, plasma vWF and GMP-140 were all higher （P〈 0.01）, and serum NO level was lower （P〈 0.01） in cerebral infarction group. Correlation analysis showed that serum PON-1 activity was negatively correlated with serum vWF （r=-0.423, P〈 0.01）, GMP-140 （r=-0.383, P〈 0.01） and plasma ox-LDL （r=-0.339, P〈 0.01） respectively, but was positively correlated with serum NO level （r=0.205, P〈0.01）. Plasma ox-LDL was negatively correlated with serum NO level （r= - 0.223, P〈0.01）, but was positively correlated with vWF （r=0.315, P〈0.01） and GMP-140 （r=0.203, P〈0.01） respectively. Serum PON-1 activity was not related with serum HDL-C, and ApoB （P〉 0.05）. Conclusion Decreased serum PON-1 activity is associated with increased plasma ox-LDL in patients with cerebral infarction. Decreased serum PON-1 activity and increased plasma ox-LDL are the risk factors of cerebral infarction.
出处
《中国现代神经疾病杂志》
CAS
2006年第4期275-278,共4页
Chinese Journal of Contemporary Neurology and Neurosurgery
关键词
磷脂酶类
脂蛋白类
LDL
脑动脉硬化
脑梗塞
Phospholipases Lipoproteins, LDL Cerebral arteriosclerosis Cerebral infarction
