摘要
目的：探讨国人胃癌和结、直肠癌的ＡＰＣ基因突变情况。方法：采用ＰＣＲ－ＳＳＣＰ银染技术，分析了１４例胃癌和１４例散发性结、直肠癌的ＡＰＣ基因第１５外显子第６段和第７段的突变情况。结果：１４份结直肠癌原发灶样品中有１份ＡＰＣ基因第１５外显子第７段出现异常电泳条带，而１４份胃癌及其６份胃癌转移性淋巴结样品中却未观察到ＡＰＣ基因的异常电泳条带。结论：本组胃癌及淋巴结转移灶中未发现ＡＰＣ基因突变，而结、直肠癌的ＡＰＣ基因突变率为７％。
Objective:To analyse the mutations of the adenomatous polyposis coli(APC) gene in gastric and colorectal carcinomas and thereby to understand the relationship between APC mutation and aggressiveness of gastric and colorectal tumours.Methods:By polymerase chain reaction single-strand conformation polymorphisms(PCR-SSCP) analysis with silver staining,we screened 14 gastric carcinomas and 14colorectal carcinomas for mutations of the exon 15-6 and exon 15-7 segment of APC gene.Results:Mutation was detected in 1 of 14(7%) colorectal carcinomas in the segment 7 of exon 15 of APC gene whereas no abnormal mobility shift was observed in any of other tissue samples such as 14 tumours and 6 lymphatic metastasis of gastric cancer,even though SSCP analysis was extended to the segment 6 and segment 7 of the exon 15.Conclusion:These findings confirm firstly the significance of APC gene mutations of 7% in colorectal,but not gastric carcinomas or its lymphatic metastasis in Chinese.These results may contribute to the shorter segment of APC gene which was analyzed in our study.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1996年第4期347-349,共3页
Academic Journal of Second Military Medical University
关键词
胃肿瘤
结肠肿瘤
直肠肿瘤
APC基因
基因突变
genes,tumour suppressor
neoplasms,stomach
neoplasms,colorectal
neoplasm metastasis
mutation,gene
PCR
