摘要
应用国产白介素２（ＩＬ－２）与骨髓细胞共同孵育１或３天，产生激活骨髓（分别记为ＡＢＭ１或ＡＢＭ３）。用３Ｈ－ＴｄＲ释放法测定，表明ＡＢＭ对肿瘤细胞株ＡＧＺＹ－８３ａ和ＣＥＭ具有明显杀伤活性。ＡＢＭ３抗瘤活性明显高于ＡＢＭ１，而新鲜骨髓或单用ＩＬ－２不具有明显抗肿瘤活性。ＡＢＭ可以净化自身骨髓中的ＣＥＭ细胞（效／靶为５０／１），而ＡＢＭ与对照组骨髓比较粒单集落形成率无统计学差别，提示ＡＢＭ仍然保持其造血活性。本文结果初步提示ＡＢＭ可用于净化骨髓中肿瘤细胞之目的。
Human bone marrow cells were incubated with IL-2 for 1 or 3 days to generate activated bone marrow(ABM)cells.3H-TdR release assay was used for tumoricidal activity appraisal.The results showed that the cytotoxicity of ABM cells against tumor cell line AGZY-83a and CEM was remarkable.The cytotoxicity of bone marrow after IL-2 activation for 3 days was much stronger than that of 1 day activation. Fresh bone marrow(FBM) alone or IL-2 alone had no influence on the anti-tumor activity.FBM contaminated with CEM cells(50/1)and treated with IL-2 showed significant ability to purge autologous CEM cells.Colonogenic potential(CFU-GM) of ABM was not impaired compared with FBM.This study suggested that bone marrow can be activated with IL-2 to acquire the ability to eliminate contaminating tumor cells without affecting its progenitor cell function in vitro.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
1996年第12期845-848,共4页
Chinese Journal of Clinical Oncology
基金
辽宁省教委重点科研基金
关键词
骨髓净化
抗肿瘤作用
骨髓移植
白细胞介素2
Interleukin-2 Activated bone marrow Purging of bone marrow Antitumor response
