摘要
目的研究卡托普利和缬沙坦对心肌梗死(MI)后胶原网络(cardiac collagen net-work,CCN)重塑的作用及作用机制。方法SD大鼠29只,建立心肌梗死模型,随机分为3组,分别为单纯心肌梗死组(MI)、卡托普利组(Cap)和缬沙坦组(Val),另设假手术组(S)不结扎冠状动脉。观察大鼠心肌羟脯氨酸含量(HC);采用RT-PCR方法检测心肌营养素-1(CT-1)mRNA和糖蛋白gp130基因(gp130)mRNA的表达。结果(1)与S组相比,MI组、Cap组和Val组心肌组织HC明显增加(P<0.01),差异有统计学意义;与MI组相比,Cap组和Val组非梗死区(NIZ)心肌组织HC均明显降低(P<0.01),差异有统计学意义。(2)与S组相比,MI组、Cap组和Val组心肌CT-1及gp130 mRNA表达增强(P<0.01),差异有统计学意义;与MI组相比,Val组CT-1及gp130 mRNA表达均显著减弱(P<0.05),差异有统计学意义;Cap组gp130mRNA表达显著减弱(P<0.05),差异有统计学意义,CT-1 mRNA差异无统计学意义(P>0.05)。结论大鼠MI后CT-1及gp130 mRNA的过度表达与MI后CCN重塑的发生密切相关;缬沙坦改善MI后CCN重塑的机制还可能与抑制CT-1及gp130基因的过度表达有关,值得进一步研究。
Objective To study the effects of captopril and valsartan on cardiac collagen net-work remodeling. Methods Twen- ty-nine SD rats were randomly divided into three groups;infracted group, captopril group and valsartan group. Except sham group, left anterior decending coronary artery was ligated. Hydroxyproline concerning collagen remodeling were observed and CT-1 mRNA and gp130 mRNA expression was tested by reverse transcription and polymerase chain reaction（RT-PCR）. Results （1） HC was increased in infracted group, captopril and valsartan group compared with those in sham group. NIZ HC was decreased in captopril and valsartan group compared with infracted group（P〈0.05 or 0.01）. （2）NIZ CT-1 mRNA and gp130 mRNA expression were increased in infracted group compared with those in sham group（P〈0.01）. In valsartan group, expressions were remarkably decreased than in infracted group（P〈0.05）. In captopril group CT-1 mRNA had no obvious chance（P〈0.05）and gp130 mRNA expression was decreased（P〈0.05）. Conclusion Overexpression of CT-1 and gp130 may play an important role in CCN remode- ling after MI, the mechanisms of valsartan preventing CCN remodeling may be partly through depressing CT-1 and gp130 overex- pression, which is worth studying further.
出处
《重庆医学》
CAS
CSCD
2006年第15期1375-1377,共3页
Chongqing medicine
