摘要
为研究二甲基甲酰胺（ＤＭＦ）的免疫毒性，从免疫器官病理组织学检查、足垫肿胀实验、外周血淋巴细胞α－醋酸萘酯酶（ＡＮＡＥ）染色，脾细胞Ⅱ型白细胞介素（ＩＬ－２）释放，体外刀豆素Ａ（ＣｏｎＡ）诱导的淋巴细胞增殖等四个方面研究了ＤＭＦ对动物免疫器官及细胞介导免疫的影响。结果显示，ＤＭＦ可使脾脏、胸腺相对重量减轻，绵羊红细胞介导的足垫反应值明显降低。皮下注射及灌胃染毒可使不同种属动物的ＡＮＡＥ阳性率降低，在１／１０～１／１６０ＬＤ５０情况下还可使ＣｏｎＡ诱导的ＩＬ－２释放明显减少。体外试验显示，ＤＭＦ在１０～１５０ｍｍｏｌ／Ｌ之间可明显抑制ＣｏｎＡ诱导的淋巴细胞增殖，表明ＤＭＦ对小鼠具有细胞免疫毒性作用。
Pathologicalexaminationoftheimmuneorgans,pedalpadswelingexperiments,α-acetatenaphthalolesterasestainforperipheralbloodlymphocyte,spleencelinterleukin-2(IL-2)re-lease,invitroconcanavalin-A(Con-A)-inducedlymphocyteproliferationtestwerecarriedouttostudytheeffectsofdimethylformamide(DMF)ontheanimalimmuneorgansanditscelularim-munotoxicity.ResultsshowedDMFcouldlowertherelativeweightofspleenandthymusandde-creasesignificantlyreactionvalueofpedalpadswelingmediatedbysheepredbloodcelinmice.Ex-posuretoDMFthroughsubcutaneousinjectionandgastricinstilationcouldcausethedecreaseofpe-ripheralANAE-positivelymphocyteindifferentspeciesofanimals.ExposuretoDMFatdosesof1/10and1/160LD50couldsignificantlycausedecreaseofIL-2release.TestsinvitroshowedDMFwithdosesof10～150mmol/LcouldinhibitsignificantlylymphocyteproliferationinducedbyCon-A.ItsuggestsDMFhasefectsoncelularimmunotoxicityinmice.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
1996年第5期269-272,共4页
Chinese Journal of Preventive Medicine
