摘要
以无刺红树莓带芽茎段和茎尖为外植体,筛选各组织培养阶段高频、稳定的适宜培养基。结果表明:外植体以茎段为佳。分化培养基以1/2 MS+6-BA 1.0 mg/L+活性炭0.1%最好,分化率可达100%;继代增殖培养基以MS+6-BA 1.0 mg/L+NAA 0.1 mg/L最理想,增殖倍数可达7倍;生根培养基以1/2 MS+IBA1.0 mg/L+活性炭1.0 g/L最佳,生根率可达93.33%。生根类型多为皮部生根。采用2步移栽法进行移裁时,第1次移裁以河砂为基质移栽成活率达到98%,第2次移裁成活率100%。
Using stem segments with buds and tips as explants, tube rapid propagation of Red Raspberry was studied. The results indicated that the appropriate media for respective culture stages were： for germination of auxiliary buds： 1/2 MS ＋ 6-BA 1.0 nag/L; for differentiation and multiplication： MS ＋ 6-BA 1.0 rag/L＋ NAA 0.1 rag/L, the multiple for multiplication was up to 7; for rooting： 1/2MS ＋ IBA 1.0 nag/L＋ active carbon 1.0 g/L and the rate of rooting was 93.33%. After 20-day acclimatization, the rooted plantlets were transplanted to substrata in two successive steps, the survival rates of which were 98% and 100% respectively.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2006年第4期411-414,共4页
Journal of Jilin Agricultural University
基金
国家自然科学基金资助项目(30560091)
关键词
无刺红树莓
茎段
组织培养
激素
thomless red raspberry
stem
tissue culture
hormone
